Please use this identifier to cite or link to this item:
http://doi.org/10.25358/openscience-9096
Authors: | Korkmaz, Yüksel Plomann, Markus Puladi, Behrus Demirbas, Aysegül Bloch, Wilhelm Deschner, James |
Title: | Dental pulp inflammation initiates the occurrence of mast cells expressing the α1 and β1 subunits of soluble guanylyl cyclase |
Online publication date: | 22-May-2023 |
Year of first publication: | 2023 |
Language: | english |
Abstract: | The binding of nitric oxide (NO) to heme in the β1 subunit of soluble guanylyl cyclase (sGC) activates both the heterodimeric α1β1 and α2β1 isoforms of the enzyme, leading to the increased production of cGMP from GTP. In cultured human mast cells, exogenous NO is able to inhibit mast cell degranulation via NO-cGMP signaling. However, under inflammatory oxidative or nitrosative stress, sGC becomes insensitive to NO. The occurrence of mast cells in healthy and inflamed human tissues and the in vivo expression of the α1 and β1 subunits of sGC in human mast cells during inflammation remain largely unresolved and were investigated here. Using peroxidase and double immunohistochemical incubations, no mast cells were found in healthy dental pulp, whereas the inflammation of dental pulp initiated the occurrence of several mast cells expressing the α1 and β1 subunits of sGC. Since inflammation-induced oxidative and nitrosative stress oxidizes Fe2+ to Fe3+ in the β1 subunit of sGC, leading to the desensitization of sGC to NO, we hypothesize that the NO- and heme-independent pharmacological activation of sGC in mast cells may be considered as a regulatory strategy for mast cell functions in inflamed human dental pulp. |
DDC: | 610 Medizin 610 Medical sciences |
Institution: | Johannes Gutenberg-Universität Mainz |
Department: | FB 04 Medizin |
Place: | Mainz |
ROR: | https://ror.org/023b0x485 |
DOI: | http://doi.org/10.25358/openscience-9096 |
Version: | Published version |
Publication type: | Zeitschriftenaufsatz |
Document type specification: | Scientific article |
License: | CC BY |
Information on rights of use: | https://creativecommons.org/licenses/by/4.0/ |
Journal: | International journal of molecular sciences 24 2 |
Pages or article number: | 901 |
Publisher: | Molecular Diversity Preservation International |
Publisher place: | Basel |
Issue date: | 2023 |
ISSN: | 1422-0067 |
Publisher DOI: | 10.3390/ijms24020901 |
Appears in collections: | DFG-491381577-G |
Files in This Item:
File | Description | Size | Format | ||
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dental_pulp_inflammation_init-20230516134734269.pdf | 5.1 MB | Adobe PDF | View/Open |