Dental pulp inflammation initiates the occurrence of mast cells expressing the α1 and β1 subunits of soluble guanylyl cyclase

dc.contributor.authorKorkmaz, Yüksel
dc.contributor.authorPlomann, Markus
dc.contributor.authorPuladi, Behrus
dc.contributor.authorDemirbas, Aysegül
dc.contributor.authorBloch, Wilhelm
dc.contributor.authorDeschner, James
dc.date.accessioned2023-05-22T08:39:51Z
dc.date.available2023-05-22T08:39:51Z
dc.date.issued2023
dc.description.abstractThe binding of nitric oxide (NO) to heme in the β1 subunit of soluble guanylyl cyclase (sGC) activates both the heterodimeric α1β1 and α2β1 isoforms of the enzyme, leading to the increased production of cGMP from GTP. In cultured human mast cells, exogenous NO is able to inhibit mast cell degranulation via NO-cGMP signaling. However, under inflammatory oxidative or nitrosative stress, sGC becomes insensitive to NO. The occurrence of mast cells in healthy and inflamed human tissues and the in vivo expression of the α1 and β1 subunits of sGC in human mast cells during inflammation remain largely unresolved and were investigated here. Using peroxidase and double immunohistochemical incubations, no mast cells were found in healthy dental pulp, whereas the inflammation of dental pulp initiated the occurrence of several mast cells expressing the α1 and β1 subunits of sGC. Since inflammation-induced oxidative and nitrosative stress oxidizes Fe2+ to Fe3+ in the β1 subunit of sGC, leading to the desensitization of sGC to NO, we hypothesize that the NO- and heme-independent pharmacological activation of sGC in mast cells may be considered as a regulatory strategy for mast cell functions in inflamed human dental pulp.en_GB
dc.description.sponsorshipDeutsche Forschungsgemeinschaft (DFG)|491381577|Open-Access-Publikationskosten 2022–2024 Universität Mainz - Universitätsmedizin
dc.identifier.doihttp://doi.org/10.25358/openscience-9096
dc.identifier.urihttps://openscience.ub.uni-mainz.de/handle/20.500.12030/9113
dc.language.isoengde
dc.rightsCC-BY-4.0*
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/*
dc.subject.ddc610 Medizinde_DE
dc.subject.ddc610 Medical sciencesen_GB
dc.titleDental pulp inflammation initiates the occurrence of mast cells expressing the α1 and β1 subunits of soluble guanylyl cyclaseen_GB
dc.typeZeitschriftenaufsatzde
jgu.journal.issue2de
jgu.journal.titleInternational journal of molecular sciencesde
jgu.journal.volume24de
jgu.organisation.departmentFB 04 Medizinde
jgu.organisation.nameJohannes Gutenberg-Universität Mainz
jgu.organisation.number2700
jgu.organisation.placeMainz
jgu.organisation.rorhttps://ror.org/023b0x485
jgu.pages.alternative901de
jgu.publisher.doi10.3390/ijms24020901de
jgu.publisher.issn1422-0067de
jgu.publisher.nameMolecular Diversity Preservation Internationalde
jgu.publisher.placeBaselde
jgu.publisher.year2023
jgu.rights.accessrightsopenAccess
jgu.subject.ddccode610de
jgu.subject.dfgLebenswissenschaftende
jgu.type.contenttypeScientific articlede
jgu.type.dinitypeArticleen_GB
jgu.type.resourceTextde
jgu.type.versionPublished versionde

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