Please use this identifier to cite or link to this item:
http://doi.org/10.25358/openscience-8072
Authors: | Siebenaller, Carmen Schlösser, Lukas Junglas, Benedikt Schmidt-Dengler, Martina Jacob, Dominik Hellmann, Nadja Sachse, Carsten Helm, Mark Schneider, Dirk |
Title: | Binding and/or hydrolysis of purine-based nucleotides is not required for IM30 ring formation |
Online publication date: | 11-Nov-2022 |
Year of first publication: | 2021 |
Language: | english |
Abstract: | IM30, the inner membrane-associated protein of 30 kDa, is conserved in cyanobacteria and chloroplasts. Although its exact physiological function is still mysterious, IM30 is clearly essential for thylakoid membrane biogenesis and/or dynamics. Recently, a cryptic IM30 GTPase activity has been reported, albeit thus far no physiological function has been attributed to this. Yet, it is still possible that GTP binding/hydrolysis affects formation of the prototypical large homo-oligomeric IM30 ring and rod structures. Here, we show that the Synechocystis sp. PCC 6803 IM30 protein in fact is an NTPase that hydrolyzes GTP and ATP, but not CTP or UTP, with about identical rates. While IM30 forms large oligomeric ring complexes, nucleotide binding and/or hydrolysis are clearly not required for ring formation. |
DDC: | 540 Chemie 540 Chemistry and allied sciences 570 Biowissenschaften 570 Life sciences |
Institution: | Johannes Gutenberg-Universität Mainz |
Department: | FB 09 Chemie, Pharmazie u. Geowissensch. |
Place: | Mainz |
ROR: | https://ror.org/023b0x485 |
DOI: | http://doi.org/10.25358/openscience-8072 |
Version: | Published version |
Publication type: | Zeitschriftenaufsatz |
License: | CC BY-NC-ND |
Information on rights of use: | https://creativecommons.org/licenses/by-nc-nd/4.0/ |
Journal: | FEBS letters 595 14 |
Pages or article number: | 1876 1885 |
Publisher: | Wiley |
Publisher place: | Chichester |
Issue date: | 2021 |
ISSN: | 1873-3468 |
Publisher DOI: | 10.1002/1873-3468.14140 |
Appears in collections: | JGU-Publikationen |
Files in This Item:
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![]() | binding_andor_hydrolysis_of_p-20221017120017531.pdf | 1.39 MB | Adobe PDF | View/Open |