Binding and/or hydrolysis of purine-based nucleotides is not required for IM30 ring formation

dc.contributor.authorSiebenaller, Carmen
dc.contributor.authorSchlösser, Lukas
dc.contributor.authorJunglas, Benedikt
dc.contributor.authorSchmidt-Dengler, Martina
dc.contributor.authorJacob, Dominik
dc.contributor.authorHellmann, Nadja
dc.contributor.authorSachse, Carsten
dc.contributor.authorHelm, Mark
dc.contributor.authorSchneider, Dirk
dc.date.accessioned2022-11-11T08:37:20Z
dc.date.available2022-11-11T08:37:20Z
dc.date.issued2021
dc.description.abstractIM30, the inner membrane-associated protein of 30 kDa, is conserved in cyanobacteria and chloroplasts. Although its exact physiological function is still mysterious, IM30 is clearly essential for thylakoid membrane biogenesis and/or dynamics. Recently, a cryptic IM30 GTPase activity has been reported, albeit thus far no physiological function has been attributed to this. Yet, it is still possible that GTP binding/hydrolysis affects formation of the prototypical large homo-oligomeric IM30 ring and rod structures. Here, we show that the Synechocystis sp. PCC 6803 IM30 protein in fact is an NTPase that hydrolyzes GTP and ATP, but not CTP or UTP, with about identical rates. While IM30 forms large oligomeric ring complexes, nucleotide binding and/or hydrolysis are clearly not required for ring formation.en_GB
dc.identifier.doihttp://doi.org/10.25358/openscience-8072
dc.identifier.urihttps://openscience.ub.uni-mainz.de/handle/20.500.12030/8087
dc.language.isoengde
dc.rightsCC-BY-NC-ND-4.0*
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/4.0/*
dc.subject.ddc540 Chemiede_DE
dc.subject.ddc540 Chemistry and allied sciencesen_GB
dc.subject.ddc570 Biowissenschaftende_DE
dc.subject.ddc570 Life sciencesen_GB
dc.titleBinding and/or hydrolysis of purine-based nucleotides is not required for IM30 ring formationen_GB
dc.typeZeitschriftenaufsatzde
jgu.journal.issue14de
jgu.journal.titleFEBS lettersde
jgu.journal.volume595de
jgu.organisation.departmentFB 09 Chemie, Pharmazie u. Geowissensch.de
jgu.organisation.nameJohannes Gutenberg-Universität Mainz
jgu.organisation.number7950
jgu.organisation.placeMainz
jgu.organisation.rorhttps://ror.org/023b0x485
jgu.pages.end1885de
jgu.pages.start1876de
jgu.publisher.doi10.1002/1873-3468.14140de
jgu.publisher.issn1873-3468de
jgu.publisher.nameWileyde
jgu.publisher.placeChichesterde
jgu.publisher.year2021
jgu.rights.accessrightsopenAccess
jgu.subject.ddccode540de
jgu.subject.ddccode570de
jgu.type.dinitypeArticleen_GB
jgu.type.resourceTextde
jgu.type.versionPublished versionde

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