Please use this identifier to cite or link to this item: http://doi.org/10.25358/openscience-5818
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dc.contributor.authorBlatt, Sebastian-
dc.contributor.authorBurckhardt, Valentin-
dc.contributor.authorKämmerer, Peer W.-
dc.contributor.authorPabst, Andreas M.-
dc.contributor.authorSagheb, Keyvan-
dc.contributor.authorHeller, Martin-
dc.contributor.authorAl-Nawas, Bilal-
dc.contributor.authorSchiegnitz, Eik-
dc.date.accessioned2021-05-04T07:38:23Z-
dc.date.available2021-05-04T07:38:23Z-
dc.date.issued2020-
dc.identifier.urihttps://openscience.ub.uni-mainz.de/handle/20.500.12030/5827-
dc.description.abstractOBJECTIVES: Porcine-derived collagen matrices (CM) can be used for oral tissue regeneration, but sufficient revascularization is crucial. The aim of this study was to analyze the influence of platelet-rich fibrin (PRF) on angiogenesis of different CM in vitro and in vivo. MATERIALS AND METHODS: Three different CM (mucoderm®, jason®, collprotect®) were combined with PRF in a plotting process. Growth factor release (VEGF, TGF-β) was measured in vitro via ELISA quantification after 1,4 and 7 days in comparison to PRF alone. In ovo yolk sac (YSM) and chorion allantois membrane (CAM) model, angiogenic potential were analyzed in vivo with light- and intravital fluorescence microscopy after 24 h, then verified with immunohistochemical staining for CD105 and αSMA. RESULTS: Highest growth factor release was seen after 24 h for all three activated membranes in comparison to the native CM (VEGF 24 h: each p < 0.05; TGF-β: each p < 0.001) and the PRF (no significant difference). All activated membranes revealed a significantly increased angiogenic potential in vivo after 24 h (vessels per mm2: each p < 0.05; branching points per mm2: each p <0.01; vessel density: each p < 0.05) and with immunohistochemical staining for CD105 (each p < 0.01) and αSMA (each p < 0.05). CONCLUSIONS: PRF improved the angiogenesis of CM in vitro and in vivo. CLINICAL RELEVANCE: Bio-functionalization of CM with PRF could easily implemented in the clinical pathway and may lead to advanced soft tissue healing.en_GB
dc.language.isoengde
dc.rightsCC BY*
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/*
dc.subject.ddc610 Medizinde_DE
dc.subject.ddc610 Medical sciencesen_GB
dc.titleBiofunctionalization of porcine-derived collagen matrices with platelet rich fibrin : influence on angiogenesis in vitro and in vivoen_GB
dc.typeZeitschriftenaufsatzde
dc.identifier.doihttp://doi.org/10.25358/openscience-5818-
jgu.type.dinitypearticleen_GB
jgu.type.versionPublished versionde
jgu.type.resourceTextde
jgu.organisation.departmentFB 04 Medizinde
jgu.organisation.number2700-
jgu.organisation.nameJohannes Gutenberg-Universität Mainz-
jgu.rights.accessrightsopenAccess-
jgu.journal.titleClinical oral investigationsde
jgu.journal.volume24de
jgu.pages.start3425de
jgu.pages.end3436de
jgu.publisher.year2020-
jgu.publisher.nameSpringerde
jgu.publisher.placeBerlin u.a.de
jgu.publisher.urihttps://doi.org/10.1007/s00784-020-03213-8de
jgu.publisher.issn1436-3771de
jgu.organisation.placeMainz-
jgu.subject.ddccode610de
jgu.publisher.doi10.1007/s00784-020-03213-8
jgu.organisation.rorhttps://ror.org/023b0x485
Appears in collections:JGU-Publikationen

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