Please use this identifier to cite or link to this item:
http://doi.org/10.25358/openscience-5818
Authors: | Blatt, Sebastian Burckhardt, Valentin Kämmerer, Peer W. Pabst, Andreas M. Sagheb, Keyvan Heller, Martin Al-Nawas, Bilal Schiegnitz, Eik |
Title: | Biofunctionalization of porcine-derived collagen matrices with platelet rich fibrin : influence on angiogenesis in vitro and in vivo |
Online publication date: | 4-May-2021 |
Year of first publication: | 2020 |
Language: | english |
Abstract: | OBJECTIVES: Porcine-derived collagen matrices (CM) can be used for oral tissue regeneration, but sufficient revascularization is crucial. The aim of this study was to analyze the influence of platelet-rich fibrin (PRF) on angiogenesis of different CM in vitro and in vivo. MATERIALS AND METHODS: Three different CM (mucoderm®, jason®, collprotect®) were combined with PRF in a plotting process. Growth factor release (VEGF, TGF-β) was measured in vitro via ELISA quantification after 1,4 and 7 days in comparison to PRF alone. In ovo yolk sac (YSM) and chorion allantois membrane (CAM) model, angiogenic potential were analyzed in vivo with light- and intravital fluorescence microscopy after 24 h, then verified with immunohistochemical staining for CD105 and αSMA. RESULTS: Highest growth factor release was seen after 24 h for all three activated membranes in comparison to the native CM (VEGF 24 h: each p < 0.05; TGF-β: each p < 0.001) and the PRF (no significant difference). All activated membranes revealed a significantly increased angiogenic potential in vivo after 24 h (vessels per mm2: each p < 0.05; branching points per mm2: each p <0.01; vessel density: each p < 0.05) and with immunohistochemical staining for CD105 (each p < 0.01) and αSMA (each p < 0.05). CONCLUSIONS: PRF improved the angiogenesis of CM in vitro and in vivo. CLINICAL RELEVANCE: Bio-functionalization of CM with PRF could easily implemented in the clinical pathway and may lead to advanced soft tissue healing. |
DDC: | 610 Medizin 610 Medical sciences |
Institution: | Johannes Gutenberg-Universität Mainz |
Department: | FB 04 Medizin |
Place: | Mainz |
ROR: | https://ror.org/023b0x485 |
DOI: | http://doi.org/10.25358/openscience-5818 |
Version: | Published version |
Publication type: | Zeitschriftenaufsatz |
License: | CC BY |
Information on rights of use: | https://creativecommons.org/licenses/by/4.0/ |
Journal: | Clinical oral investigations 24 |
Pages or article number: | 3425 3436 |
Publisher: | Springer |
Publisher place: | Berlin u.a. |
Issue date: | 2020 |
ISSN: | 1436-3771 |
Publisher URL: | https://doi.org/10.1007/s00784-020-03213-8 |
Publisher DOI: | 10.1007/s00784-020-03213-8 |
Appears in collections: | JGU-Publikationen |
Files in This Item:
File | Description | Size | Format | ||
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blatt_sebastian-biofunctionali-20210423120258389.pdf | 1.67 MB | Adobe PDF | View/Open |