Please use this identifier to cite or link to this item:
http://doi.org/10.25358/openscience-5782
Authors: | Blatt, Sebastian Pabst, Andreas Zimmer, Stefanie Walter, Christian Al-Nawas, Bilal Krüger, Maximilian |
Title: | Clinical efficacy of an antibody-based detection system for human papilloma virus infection in oral squamous cell carcinoma |
Online publication date: | 3-May-2021 |
Year of first publication: | 2021 |
Language: | english |
Abstract: | OBJECTIVES There is an increasing number of oral squamous cell carcinoma (OSCC) associated with HPV-16. However, p16 expression by immunohistochemistry as the current gold standard for a surrogate marker for virus infection reveals unsatisfying diagnostic accuracy. The aim of this study was to investigate a new rapid test for L1 antibody detection (Prevocheck®) and to validate its diagnostic performance. MATERIALS AND METHODS In a prospective study, the HPV 16 association of all consecutive patients with an OSCC treated between 2015 and 2019 were analyzed by L1 seropositivity (via PrevoCheck®), p16 immunostaining, and partly multiplex PCR for subtype analysis. RESULTS Overall (n = 107), p16 expression was positive in 17 cases (15.9%), and L1 antibody seropositivity in 7 cases (6.5%). In PCR analysis, two cases of HPV35 and 50 were found. Total HPV prevalence was 8.4% overall and 6.5% for HPV-16. An inferior diagnostic accuracy for HPV-16-associated OSCC in comparison to PrevoCheck® was revealed. CONCLUSION The rapid test for L1 antibodies showed an optimal sensitivity and specificity, positive and negative predictive value, and an overall diagnostic accuracy of 100%. However, HPV prevalence seems low in OSCC. CLINICAL RELEVANCE L1 rapid test may represent an additional diagnostic staging method to detect HPV-16 association rather than p16 immunohistochemistry. |
DDC: | 610 Medizin 610 Medical sciences |
Institution: | Johannes Gutenberg-Universität Mainz |
Department: | FB 04 Medizin |
Place: | Mainz |
ROR: | https://ror.org/023b0x485 |
DOI: | http://doi.org/10.25358/openscience-5782 |
Version: | Published version |
Publication type: | Zeitschriftenaufsatz |
License: | CC BY |
Information on rights of use: | https://creativecommons.org/licenses/by/4.0/ |
Journal: | Clinical oral investigations 25 |
Pages or article number: | 2837 2843 |
Publisher: | Springer |
Publisher place: | Berlin u.a. |
Issue date: | 2021 |
ISSN: | 1436-3771 |
Publisher URL: | https://doi.org/10.1007/s00784-020-03601-0 |
Publisher DOI: | 10.1007/s00784-020-03601-0 |
Appears in collections: | JGU-Publikationen |
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File | Description | Size | Format | ||
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blatt_sebastian-clinical_effic-20210503120346359.pdf | 706.44 kB | Adobe PDF | View/Open |