Clinical efficacy of an antibody-based detection system for human papilloma virus infection in oral squamous cell carcinoma
| dc.contributor.author | Blatt, Sebastian | |
| dc.contributor.author | Pabst, Andreas | |
| dc.contributor.author | Zimmer, Stefanie | |
| dc.contributor.author | Walter, Christian | |
| dc.contributor.author | Al-Nawas, Bilal | |
| dc.contributor.author | Krüger, Maximilian | |
| dc.date.accessioned | 2021-05-03T09:54:54Z | |
| dc.date.available | 2021-05-03T09:54:54Z | |
| dc.date.issued | 2021 | |
| dc.description.abstract | OBJECTIVES There is an increasing number of oral squamous cell carcinoma (OSCC) associated with HPV-16. However, p16 expression by immunohistochemistry as the current gold standard for a surrogate marker for virus infection reveals unsatisfying diagnostic accuracy. The aim of this study was to investigate a new rapid test for L1 antibody detection (Prevocheck®) and to validate its diagnostic performance. MATERIALS AND METHODS In a prospective study, the HPV 16 association of all consecutive patients with an OSCC treated between 2015 and 2019 were analyzed by L1 seropositivity (via PrevoCheck®), p16 immunostaining, and partly multiplex PCR for subtype analysis. RESULTS Overall (n = 107), p16 expression was positive in 17 cases (15.9%), and L1 antibody seropositivity in 7 cases (6.5%). In PCR analysis, two cases of HPV35 and 50 were found. Total HPV prevalence was 8.4% overall and 6.5% for HPV-16. An inferior diagnostic accuracy for HPV-16-associated OSCC in comparison to PrevoCheck® was revealed. CONCLUSION The rapid test for L1 antibodies showed an optimal sensitivity and specificity, positive and negative predictive value, and an overall diagnostic accuracy of 100%. However, HPV prevalence seems low in OSCC. CLINICAL RELEVANCE L1 rapid test may represent an additional diagnostic staging method to detect HPV-16 association rather than p16 immunohistochemistry. | en_GB |
| dc.identifier.doi | http://doi.org/10.25358/openscience-5782 | |
| dc.identifier.uri | https://openscience.ub.uni-mainz.de/handle/20.500.12030/5791 | |
| dc.language.iso | eng | de |
| dc.rights | CC-BY-4.0 | * |
| dc.rights.uri | https://creativecommons.org/licenses/by/4.0/ | * |
| dc.subject.ddc | 610 Medizin | de_DE |
| dc.subject.ddc | 610 Medical sciences | en_GB |
| dc.title | Clinical efficacy of an antibody-based detection system for human papilloma virus infection in oral squamous cell carcinoma | en_GB |
| dc.type | Zeitschriftenaufsatz | de |
| jgu.journal.title | Clinical oral investigations | de |
| jgu.journal.volume | 25 | de |
| jgu.organisation.department | FB 04 Medizin | de |
| jgu.organisation.name | Johannes Gutenberg-Universität Mainz | |
| jgu.organisation.number | 2700 | |
| jgu.organisation.place | Mainz | |
| jgu.organisation.ror | https://ror.org/023b0x485 | |
| jgu.pages.end | 2843 | de |
| jgu.pages.start | 2837 | de |
| jgu.publisher.doi | 10.1007/s00784-020-03601-0 | |
| jgu.publisher.issn | 1436-3771 | de |
| jgu.publisher.name | Springer | de |
| jgu.publisher.place | Berlin u.a. | de |
| jgu.publisher.uri | https://doi.org/10.1007/s00784-020-03601-0 | de |
| jgu.publisher.year | 2021 | |
| jgu.rights.accessrights | openAccess | |
| jgu.subject.ddccode | 610 | de |
| jgu.type.dinitype | Article | en_GB |
| jgu.type.resource | Text | de |
| jgu.type.version | Published version | de |