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http://doi.org/10.25358/openscience-187| Authors: | Butzbach, Kathrin Konhäuser, Matthias Fach, Matthias Bamberger, Denise N. Breitenbach, Benjamin Epe, Bernd Wich, Peter |
| Title: | Receptor-mediated uptake of folic acid-functionalized dextran nanoparticles for applications in photodynamic therapy |
| Online publication date: | 14-Aug-2019 |
| Year of first publication: | 2019 |
| Language: | english |
| Abstract: | In photodynamic therapy (PDT), photosensitizers and light are used to cause photochemically induced cell death. The selectivity and the effectiveness of the phototoxicity in cancer can be increased by a specific uptake of the photosensitizer into tumor cells. A promising target for this goal is the folic acid receptor α (FRα), which is overexpressed on the surface of many tumor cells and mediates an endocytotic uptake. Here, we describe a polysaccharide-based nanoparticle system suitable for targeted uptake and its photochemical and photobiological characterization. The photosensitizer 5, 10, 15, 20-tetraphenyl-21H, 23H-porphyrine (TPP) was encapsulated in spermine- and acetal-modified dextran (SpAcDex) nanoparticles and conjugated with folic acid (FA) on the surface [SpAcDex(TPP)-FA]. The particles are successfully taken up by human HeLa-KB cells, and a light-induced cytotoxicity is observable. An excess of free folate as the competitor for the FRα-mediated uptake inhibits the phototoxicity. In conclusion, folate-modified SpAcDex particles are a promising drug delivery system for a tumor cell targeted photodynamic therapy. |
| DDC: | 610 Medizin 610 Medical sciences |
| Institution: | Johannes Gutenberg-Universität Mainz |
| Department: | FB 09 Chemie, Pharmazie u. Geowissensch. |
| Place: | Mainz |
| ROR: | https://ror.org/023b0x485 |
| DOI: | http://doi.org/10.25358/openscience-187 |
| URN: | urn:nbn:de:hebis:77-publ-591788 |
| Version: | Published version |
| Publication type: | Zeitschriftenaufsatz |
| License: | CC BY |
| Information on rights of use: | https://creativecommons.org/licenses/by/4.0/ |
| Journal: | Polymers 11 5 |
| Pages or article number: | Art. 896 |
| Publisher: | MDPI |
| Publisher place: | Basel |
| Issue date: | 2019 |
| ISSN: | 2073-4360 |
| Publisher URL: | http://dx.doi.org/10.3390/polym11050896 |
| Publisher DOI: | 10.3390/polym11050896 |
| Appears in collections: | JGU-Publikationen |
