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Authors: Butzbach, Kathrin
Konhäuser, Matthias
Fach, Matthias
Bamberger, Denise N.
Breitenbach, Benjamin
Epe, Bernd
Wich, Peter
Title: Receptor-mediated uptake of folic acid-functionalized dextran nanoparticles for applications in photodynamic therapy
Online publication date: 14-Aug-2019
Year of first publication: 2019
Language: english
Abstract: In photodynamic therapy (PDT), photosensitizers and light are used to cause photochemically induced cell death. The selectivity and the effectiveness of the phototoxicity in cancer can be increased by a specific uptake of the photosensitizer into tumor cells. A promising target for this goal is the folic acid receptor α (FRα), which is overexpressed on the surface of many tumor cells and mediates an endocytotic uptake. Here, we describe a polysaccharide-based nanoparticle system suitable for targeted uptake and its photochemical and photobiological characterization. The photosensitizer 5, 10, 15, 20-tetraphenyl-21H, 23H-porphyrine (TPP) was encapsulated in spermine- and acetal-modified dextran (SpAcDex) nanoparticles and conjugated with folic acid (FA) on the surface [SpAcDex(TPP)-FA]. The particles are successfully taken up by human HeLa-KB cells, and a light-induced cytotoxicity is observable. An excess of free folate as the competitor for the FRα-mediated uptake inhibits the phototoxicity. In conclusion, folate-modified SpAcDex particles are a promising drug delivery system for a tumor cell targeted photodynamic therapy.
DDC: 610 Medizin
610 Medical sciences
Institution: Johannes Gutenberg-Universität Mainz
Department: FB 09 Chemie, Pharmazie u. Geowissensch.
Place: Mainz
URN: urn:nbn:de:hebis:77-publ-591788
Version: Published version
Publication type: Zeitschriftenaufsatz
License: CC BY
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Journal: Polymers
Pages or article number: Art. 896
Publisher: MDPI
Publisher place: Basel
Issue date: 2019
ISSN: 2073-4360
Publisher URL:
Publisher DOI: 10.3390/polym11050896
Appears in collections:JGU-Publikationen

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