Protocol for MR1-ligand cross-linking to discover metabolite antigens by proteomics

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dc.contributor.authorThomas, Hannah
dc.contributor.authorDolton, Garry
dc.contributor.authorCardo, Lucia F.
dc.contributor.authorTernette, Nicola
dc.contributor.authorSewell, Andrew K.
dc.contributor.authorSchmidlin, Thierry
dc.date.accessioned2026-08-04T07:22:34Z
dc.date.issued2026
dc.description.abstractHere, we present a protocol for the discovery of MR1-bound metabolite ligands by stabilizing Schiff base interactions between MR1 and its ligands. We describe steps for enriching MR1-ligand complexes using MR1-expressing A549 cells and covalently cross-linking ligands to the MR1 lysine 43 residue via reductive amination. We then detail procedures for identifying ligand-modified MR1 peptides by mass spectrometry and for further validation of candidate ligands using MR1 surface upregulation and TCR functional assays.en
dc.identifier.doihttps://doi.org/10.25358/openscience-16026
dc.identifier.urihttps://openscience.ub.uni-mainz.de/handle/20.500.12030/16047
dc.language.isoeng
dc.rightsCC-BY-4.0
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.subject.ddc610 Medizinde
dc.subject.ddc610 Medical sciencesen
dc.titleProtocol for MR1-ligand cross-linking to discover metabolite antigens by proteomics
dc.typeZeitschriftenaufsatz
jgu.apc.netprice720,00
jgu.apc.price770,40
jgu.apc.taxrate7
jgu.apc.transformationcontractElsevier
jgu.dfg.year2026
jgu.identifier.uuid45e4a135-98c8-48ff-bf13-a05585f8da95
jgu.journal.issue1
jgu.journal.titleSTAR Protocols
jgu.journal.volume7
jgu.nationalcurrency.eur720,00
jgu.organisation.departmentFB 04 Medizin
jgu.organisation.nameJohannes Gutenberg-Universität Mainz
jgu.organisation.number2700
jgu.organisation.placeMainz
jgu.organisation.rorhttps://ror.org/023b0x485
jgu.pages.alternative104399
jgu.publisher.doi10.1016/j.xpro.2026.104399
jgu.publisher.eissn2666-1667
jgu.publisher.nameCell Press
jgu.publisher.placeCambridge, Mass.
jgu.publisher.year2026
jgu.rights.accessrightsopenAccess
jgu.subject.ddccode610
jgu.subject.dfgLebenswissenschaften
jgu.type.contenttypeOther
jgu.type.dinitypeArticleen_GB
jgu.type.resourceText
jgu.type.versionPublished version

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