Authors:	Ritz, Ulrike Gerke, Rebekka Götz, Hermann Stein, Stefan Rommens, Pol Maria
Title:	A new bone substitute developed from 3D-prints of polylactide (PLA) loaded with collagen I : an in vitro study
Online publication date:	27-Feb-2018
Year of first publication:	2017
Language:	english
Abstract:	Although a lot of research has been performed, large segmental bone defects caused by trauma, infection, bone tumors or revision surgeries still represent big challenges for trauma surgeons. New and innovative bone substitutes are needed. Three-dimensional (3D) printing is a novel procedure to create 3D porous scaffolds that can be used for bone tissue engineering. In the present study, solid discs as well as porous cage-like 3D prints made of polylactide (PLA) are coated or filled with collagen, respectively, and tested for biocompatibility and endotoxin contamination. Microscopic analyses as well as proliferation assays were performed using various cell types on PLA discs. Stromal-derived factor (SDF-1) release from cages filled with collagen was analyzed and the effect on endothelial cells tested. This study confirms the biocompatibility of PLA and demonstrates an endotoxin contamination clearly below the FDA (Food and Drug Administration) limit. Cells of various cell types (osteoblasts, osteoblast-like cells, fibroblasts and endothelial cells) grow, spread and proliferate on PLA-printed discs. PLA cages loaded with SDF-1 collagen display a steady SDF-1 release, support cell growth of endothelial cells and induce neo-vessel formation. These results demonstrate the potential for PLA scaffolds printed with an inexpensive desktop printer in medical applications, for example, in bone tissue engineering.
DDC:	610 Medizin 610 Medical sciences
Institution:	Johannes Gutenberg-Universität
Department:	FB 04 Medizin
Place:	Mainz
ROR:	https://ror.org/023b0x485
DOI:	http://doi.org/10.25358/openscience-97
URN:	urn:nbn:de:hebis:77-publ-578731
Version:	Published version
Publication type:	Zeitschriftenaufsatz
License:	CC BY
Information on rights of use:	https://creativecommons.org/licenses/by/4.0/
Journal:	International journal of molecular sciences 18 12
Pages or article number:	Art. 2569
Publisher:	Molecular Diversity Preservation International
Publisher place:	Basel
Issue date:	2017
ISSN:	1422-0067 1661-6596
Publisher URL:	http://dx.doi.org/10.3390/ijms18122569
Publisher DOI:	10.3390/ijms18122569
Appears in collections:	JGU-Publikationen