A new 3D-printed polylactic acid-bioglass composite for bone tissue engineering induces angiogenesis in vitro and in ovo
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Abstract
Large bone defects such as those that occur after trauma or resections due to cancer
still are a challenge for surgeons. Main challenge in this area is to find a suitable
alternative to the gold-standard therapy, which is highly risky, and a promising option is
to use biomaterials manufactured by 3D printing. In former studies, we demonstrated
that the combination of polylactic acid (PLA) and bioglass (BG) resulted in a stable
3D-printable material, and porous and finely structured scaffolds were printed. These
scaffolds exhibited osteogenic and anti-inflammatory properties. This 3D-printed
material fulfills most of the requirements described in the diamond concept of bone
healing. However, the question remains as to whether it also meets the requirements
concerning angiogenesis. Therefore, the aim of this study was to analyze the effects
of the 3D-printed PLA-BG composite material on angiogenesis. In vitro analyses
with human umbilical vein endothelial cells (HUVECs) showed a positive effect of
increasing BG content on viability and gene expression of endothelial markers.
This positive effect was confirmed by an enhanced vascular formation analyzed by
Matrigel assay and chicken chorioallantoic membrane (CAM) assay. In this work, we
demonstrated the angiogenic efficiency of a 3D-printed PLA–BG composite material.
Recalling the osteogenic potential of this material demonstrated in former work,
we manufactured a mechanically stable, 3D-printable, osteogenic and angiogenic
material, which could be used for bone tissue engineering.
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International journal of bioprinting, 9, 5, Whioce, Singapore, 2023, https://doi.org/10.18063/ijb.751