Please use this identifier to cite or link to this item:
http://doi.org/10.25358/openscience-9131
Authors: | Ziskoven, Pablo Cores Nogueira, Andressa V. B. Gutierrez, Lorena S. Weusmann, Jens Eick, Sigrun Buduneli, Nurcan Deschner, James |
Title: | Apelin enhances the effects of Fusobacterium nucleatum on periodontal ligament cells in vitro |
Online publication date: | 26-May-2023 |
Year of first publication: | 2023 |
Language: | english |
Abstract: | This study aimed to explore effects of Fusobacterium nucleatum with or without apelin on periodontal ligament (PDL) cells to better understand pathomechanistic links between periodontitis and obesity. First, the actions of F. nucleatum on COX2, CCL2, and MMP1 expressions were assessed. Subsequently, PDL cells were incubated with F. nucleatum in the presence and absence of apelin to study the modulatory effects of this adipokine on molecules related to inflammation and hard and soft tissue turnover. Regulation of apelin and its receptor (APJ) by F. nucleatum was also studied. F. nucleatum resulted in elevated COX2, CCL2, and MMP1 expressions in a dose- and time-dependent manner. Combination of F. nucleatum and apelin led to the highest (p < 0.05) expression levels of COX2, CCL2, CXCL8, TNF-α, and MMP1 at 48 h. The effects of F. nucleatum and/or apelin on CCL2 and MMP1 were MEK1/2- and partially NF-κB-dependent. The combined effects of F. nucleatum and apelin on CCL2 and MMP1 were also observed at protein level. Moreover, F. nucleatum downregulated (p < 0.05) the apelin and APJ expressions. In conclusion, obesity could contribute to periodontitis through apelin. The local production of apelin/APJ in PDL cells also suggests a role of these molecules in the pathogenesis of periodontitis. |
DDC: | 610 Medizin 610 Medical sciences |
Institution: | Johannes Gutenberg-Universität Mainz |
Department: | FB 04 Medizin |
Place: | Mainz |
ROR: | https://ror.org/023b0x485 |
DOI: | http://doi.org/10.25358/openscience-9131 |
Version: | Published version |
Publication type: | Zeitschriftenaufsatz |
Document type specification: | Scientific article |
License: | CC BY |
Information on rights of use: | https://creativecommons.org/licenses/by/4.0/ |
Journal: | International journal of molecular sciences 24 5 |
Pages or article number: | 4733 |
Publisher: | MDPI |
Publisher place: | Basel |
Issue date: | 2023 |
ISSN: | 1422-0067 |
Publisher DOI: | 10.3390/ijms24054733 |
Appears in collections: | DFG-491381577-G |
Files in This Item:
File | Description | Size | Format | ||
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apelin_enhances_the_effects_o-20230525154842788.pdf | 3.81 MB | Adobe PDF | View/Open |