Authors:	Butto, Tamer Mungikar, Kanak Baumann, Peter Winter, Jennifer Lutz, Beat Gerber, Susanne
Title:	Nuclei on the rise : when nuclei-based methods meet next-generation sequencing
Online publication date:	20-Apr-2023
Year of first publication:	2023
Language:	english
Abstract:	In the last decade, we have witnessed an upsurge in nuclei-based studies, particularly coupled with next-generation sequencing. Such studies aim at understanding the molecular states that exist in heterogeneous cell populations by applying increasingly more affordable sequencing approaches, in addition to optimized methodologies developed to isolate and select nuclei. Although these powerful new methods promise unprecedented insights, it is important to understand and critically consider the associated challenges. Here, we provide a comprehensive overview of the rise of nuclei-based studies and elaborate on their advantages and disadvantages, with a specific focus on their utility for transcriptomic sequencing analyses. Improved designs and appropriate use of the various experimental strategies will result in acquiring biologically accurate and meaningful information.
DDC:	570 Biowissenschaften 570 Life sciences
Institution:	Johannes Gutenberg-Universität Mainz
Department:	FB 10 Biologie
Place:	Mainz
ROR:	https://ror.org/023b0x485
DOI:	http://doi.org/10.25358/openscience-9027
Version:	Published version
Publication type:	Zeitschriftenaufsatz
Document type specification:	Scientific article
License:	CC BY
Information on rights of use:	https://creativecommons.org/licenses/by/4.0/
Journal:	Cells 12 7
Pages or article number:	1051
Publisher:	MDPI
Publisher place:	Basel
Issue date:	2023
ISSN:	2073-4409
Publisher DOI:	10.3390/cells12071051
Appears in collections:	DFG-491381577-G