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Authors: Deng, Jie
Liu, Wei
Sun, Mo
Walther, Andreas
Title: Dissipative organization of DNA oligomers for transient catalytic function
Online publication date: 3-Feb-2023
Year of first publication: 2022
Language: english
Abstract: The development of synthetic non-equilibrium systems opens doors for man-made life-like materials. Yet, creating distinct transient functions from artificial fuel-driven structures remains a challenge. Building on our ATP-driven dynamic covalent DNA assembly in an enzymatic reaction network of concurrent ATP-powered ligation and restriction, we introduce ATP-fueled transient organization of functional subunits for various functions. The programmability of the ligation/restriction site allows to precisely organize multiple sticky-end-encoded oligo segments into double-stranded (ds) DNA complexes. We demonstrate principles of ATP-driven organization into sequence-defined oligomers by sensing barcode-embedded targets with different defects. Furthermore, ATP-fueled DNAzymes for substrate cleavage are achieved by transiently ligating two DNAzyme subunits into a dsDNA complex, rendering ATP-fueled transient catalytic function.
DDC: 540 Chemie
540 Chemistry and allied sciences
Institution: Johannes Gutenberg-Universität Mainz
Department: FB 09 Chemie, Pharmazie u. Geowissensch.
Place: Mainz
Version: Published version
Publication type: Zeitschriftenaufsatz
Document type specification: Scientific article
License: CC BY-NC
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Journal: Angewandte Chemie : International edition
Pages or article number: 202113477
Publisher: Wiley-VCH
Publisher place: Weinheim
Issue date: 2022
ISSN: 1521-3773
Publisher DOI: 10.1002/anie.202113477
Appears in collections:DFG-491381577-H

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