Dissipative organization of DNA oligomers for transient catalytic function
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Abstract
The development of synthetic non-equilibrium systems opens doors for man-made life-like materials. Yet,
creating distinct transient functions from artificial fuel-driven structures remains a challenge. Building on our ATP-driven dynamic covalent DNA assembly in an enzymatic reaction network of concurrent ATP-powered ligation and restriction, we introduce ATP-fueled transient organization of functional subunits for various functions. The programmability of the ligation/restriction site allows to precisely organize multiple sticky-end-encoded oligo segments into double-stranded (ds) DNA complexes. We demonstrate principles of ATP-driven organization into sequence-defined oligomers by sensing barcode-embedded targets with different defects. Furthermore, ATP-fueled DNAzymes for substrate cleavage are
achieved by transiently ligating two DNAzyme subunits into a dsDNA complex, rendering ATP-fueled transient catalytic function.
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Angewandte Chemie : International edition, 61, 10, Wiley-VCH, Weinheim, 2022, https://doi.org/10.1002/anie.202113477