Dissipative organization of DNA oligomers for transient catalytic function

dc.contributor.authorDeng, Jie
dc.contributor.authorLiu, Wei
dc.contributor.authorSun, Mo
dc.contributor.authorWalther, Andreas
dc.date.accessioned2023-02-03T12:44:01Z
dc.date.available2023-02-03T12:44:01Z
dc.date.issued2022
dc.description.abstractThe development of synthetic non-equilibrium systems opens doors for man-made life-like materials. Yet, creating distinct transient functions from artificial fuel-driven structures remains a challenge. Building on our ATP-driven dynamic covalent DNA assembly in an enzymatic reaction network of concurrent ATP-powered ligation and restriction, we introduce ATP-fueled transient organization of functional subunits for various functions. The programmability of the ligation/restriction site allows to precisely organize multiple sticky-end-encoded oligo segments into double-stranded (ds) DNA complexes. We demonstrate principles of ATP-driven organization into sequence-defined oligomers by sensing barcode-embedded targets with different defects. Furthermore, ATP-fueled DNAzymes for substrate cleavage are achieved by transiently ligating two DNAzyme subunits into a dsDNA complex, rendering ATP-fueled transient catalytic function.en_GB
dc.description.sponsorshipGefördert durch die Deutsche Forschungsgemeinschaft (DFG) - Projektnummer 491381577de
dc.identifier.doihttp://doi.org/10.25358/openscience-8761
dc.identifier.urihttps://openscience.ub.uni-mainz.de/handle/20.500.12030/8777
dc.language.isoengde
dc.rightsCC-BY-NC-4.0*
dc.rights.urihttps://creativecommons.org/licenses/by-nc/4.0/*
dc.subject.ddc540 Chemiede_DE
dc.subject.ddc540 Chemistry and allied sciencesen_GB
dc.titleDissipative organization of DNA oligomers for transient catalytic functionen_GB
dc.typeZeitschriftenaufsatzde
jgu.journal.issue10de
jgu.journal.titleAngewandte Chemie : International editionde
jgu.journal.volume61de
jgu.organisation.departmentFB 09 Chemie, Pharmazie u. Geowissensch.de
jgu.organisation.nameJohannes Gutenberg-Universität Mainz
jgu.organisation.number7950
jgu.organisation.placeMainz
jgu.organisation.rorhttps://ror.org/023b0x485
jgu.pages.alternative202113477de
jgu.publisher.doi10.1002/anie.202113477de
jgu.publisher.issn1521-3773de
jgu.publisher.nameWiley-VCHde
jgu.publisher.placeWeinheimde
jgu.publisher.year2022
jgu.rights.accessrightsopenAccess
jgu.subject.ddccode540de
jgu.subject.dfgNaturwissenschaftende
jgu.type.contenttypeScientific articlede
jgu.type.dinitypeArticleen_GB
jgu.type.resourceTextde
jgu.type.versionPublished versionde

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