Please use this identifier to cite or link to this item:
http://doi.org/10.25358/openscience-8683
Authors: | Sohl, Julian Hartmann, Ann-Kathrin Hahlbrock, Jennifer Bartneck, Joschka Stassen, Michael Klein, Matthias Bros, Matthias Grabbe, Stephan Marini, Federico Woods, Kevin Guezguez, Borhane Mack, Matthias Schild, Hansjörg Muth, Sabine Melchior, Felix Probst, Hans Christian Langguth, Peter Radsak, Markus P. |
Title: | Dithranol as novel co-adjuvant for non-invasive dermal vaccination |
Online publication date: | 13-Feb-2023 |
Year of first publication: | 2022 |
Language: | english |
Abstract: | Transcutaneous immunization (TCI) utilizing the TLR7 agonist imiquimod (IMQ-TCI) induces T cell-driven protective immunity upon application onto intact skin. In our present work, we combine the anti-psoriatic agent dithranol with IMQ-TCI to boost vaccination efficacy (Dithranol/IMQ-based transcutaneous vaccination (DIVA)). Using ovalbumin-derived peptides as model antigens in mice, DIVA induced superior cytolytic CD8+ T cells and CD4+ T cells with a TH1 cytokine profile in the priming as well as in the memory phase. Regarding the underlying mechanisms, dithranol induced an oxidant-dependent, monocyte-attracting inflammatory milieu in the skin boosting TLR7-dependent activation of dendritic cells and macrophages leading to superior T cell priming and protective immunity in vaccinia virus infection. In conclusion, we introduce the non-invasive vaccination method DIVA to induce strong primary and memory T cell responses upon a single local treatment. This work provides relevant insights in cutaneous vaccination approaches, paving the way for clinical development in humans. |
DDC: | 610 Medizin 610 Medical sciences |
Institution: | Johannes Gutenberg-Universität Mainz |
Department: | FB 04 Medizin |
Place: | Mainz |
ROR: | https://ror.org/023b0x485 |
DOI: | http://doi.org/10.25358/openscience-8683 |
Version: | Published version |
Publication type: | Zeitschriftenaufsatz |
Document type specification: | Scientific article |
License: | CC BY |
Information on rights of use: | https://creativecommons.org/licenses/by/4.0/ |
Journal: | npj vaccines 7 |
Pages or article number: | 112 |
Publisher: | Nature Publishing Group |
Publisher place: | London |
Issue date: | 2022 |
ISSN: | 2059-0105 |
Publisher DOI: | 10.1038/s41541-022-00530-9 |
Appears in collections: | DFG-491381577-G |
Files in This Item:
File | Description | Size | Format | ||
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![]() | dithranol_as_novel_coadjuvant-20230127111116015.pdf | 2.69 MB | Adobe PDF | View/Open |