Please use this identifier to cite or link to this item:
Authors: Penner, Inessa
Büscher, Nicole
Dejung, Mario
Freiwald, Anja
Butter, Falk
Plachter, Bodo
Title: Subviral dense bodies of human cytomegalovirus induce an antiviral type I interferon response
Online publication date: 8-Feb-2023
Year of first publication: 2022
Language: english
Abstract: (1) Background: Cells infected with the human cytomegalovirus (HCMV) produce subviral particles, termed dense bodies (DBs), both in-vitro and in-vivo. They are released from cells, comparable to infectious virions, and are enclosed by a membrane that resembles the viral envelope and mediates the entry into cells. To date, little is known about how the DB uptake influences the gene expression in target cells. The purpose of this study was to investigate the impact of DBs on cells, in the absence of a viral infection. (2) Methods: Mass spectrometry, immunoblot analyses, siRNA knockdown, and a CRISPR-CAS9 knockout, were used to investigate the changes in cellular gene expression following a DB exposure; (3) Results: A number of interferon-regulated genes (IRGs) were upregulated after the fibroblasts and endothelial cells were exposed to DBs. This upregulation was dependent on the DB entry and mediated by the type I interferon signaling through the JAK-STAT pathway. The induction of IRGs was mediated by the sensing of the DB-introduced DNA by the pattern recognition receptor cGAS. (4) Conclusions: The induction of a strong type I IFN response by DBs is a unique feature of the HCMV infection. The release of DBs may serve as a danger signal and concomitantly contribute to the induction of a strong, antiviral immune response.
DDC: 610 Medizin
610 Medical sciences
Institution: Johannes Gutenberg-Universität Mainz
Department: FB 04 Medizin
Place: Mainz
Version: Published version
Publication type: Zeitschriftenaufsatz
Document type specification: Scientific article
License: CC BY
Information on rights of use:
Journal: Cells
Pages or article number: 4028
Publisher: MDPI
Publisher place: Basel
Issue date: 2022
ISSN: 2073-4409
Publisher DOI: 10.3390/cells11244028
Appears in collections:DFG-491381577-G

Files in This Item:
  File Description SizeFormat
subviral_dense_bodies_of_huma-20230126104851681.pdf3.82 MBAdobe PDFView/Open