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Authors: Trzeciak, Emily R.
Zimmer, Niklas
Gehringer, Isabelle
Stein, Lara
Graefen, Barbara
Schupp, Jonathan
Stephan, Achim
Rietz, Stephan
Prantner, Michael
Tüttenberg, Andrea
Title: Oxidative stress differentially influences the survival and metabolism of cells in the melanoma microenvironment
Online publication date: 8-Feb-2023
Year of first publication: 2022
Language: english
Abstract: The cellular composition of the tumor microenvironment, including tumor, immune, stromal, and endothelial cells, significantly influences responses to cancer therapies. In this study, we analyzed the impact of oxidative stress, induced by cold atmospheric plasma (CAP), on tumor cells, T cells, and macrophages, which comprise part of the melanoma microenvironment. To accomplish this, cells were grown in different in vitro cell culture models and were treated with varying amounts of CAP. Subsequent alterations in viability, proliferation, and phenotype were analyzed via flow cytometry and metabolic alterations by Seahorse Cell Mito Stress Tests. It was found that cells generally exhibited reduced viability and proliferation, stemming from CAP induced G2/M cell cycle arrest and subsequent apoptosis, as well as increased mitochondrial stress following CAP treatment. Overall, sensitivity to CAP treatment was found to be cell type dependent with T cells being the most affected. Interestingly, CAP influenced the polarization of M0 macrophages to a “M0/M2-like” phenotype, and M1 macrophages were found to display a heightened sensitivity to CAP induced mitochondrial stress. CAP also inhibited the growth and killed melanoma cells in 2D and 3D in vitro cell culture models in a dose-dependent manner. Improving our understanding of oxidative stress, mechanisms to manipulate it, and its implications for the tumor microenvironment may help in the discovery of new therapeutic targets.
DDC: 610 Medizin
610 Medical sciences
Institution: Johannes Gutenberg-Universität Mainz
Department: FB 04 Medizin
Place: Mainz
Version: Published version
Publication type: Zeitschriftenaufsatz
Document type specification: Scientific article
License: CC BY
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Journal: Cells
Pages or article number: 930
Publisher: MDPI
Publisher place: Basel
Issue date: 2022
ISSN: 2073-4409
Publisher DOI: 10.3390/cells11060930
Appears in collections:DFG-491381577-G

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