Autoren:	Kriegel, Anja Schlosser, Christian Habeck, Tanja Dahmen, Christoph Götz, Hermann Clauder, Franziska Armbruster, Franz Paul Baranowski, Andreas Drees, Philipp Rommens, Pol Maria Ritz, Ulrike
Titel:	Bone sialoprotein immobilized in collagen type I enhances bone regeneration in vitro and in vivo
Online-Publikationsdatum:	25-Jan-2023
Erscheinungsdatum:	2022
Sprache des Dokuments:	Englisch
Zusammenfassung/Abstract:	The use of bioactive molecules is a promising approach to enhance the bone healing properties of biomaterials. The aim of this study was to define the role of bone sialoprotein (BSP) immobilized in collagen type I in various settings. In vitro studies with human primary osteoblasts in mono- or in co-culture with endothelial cells demonstrated a slightly increased gene expression of osteogenic markers as well as an increased proliferation rate in osteoblasts after application of BSP immobilized in collagen type I. Two critical size bone defect models were used to analyze bone regeneration. BSP incorporated in collagen type I increased bone regeneration only marginally at one concentration in a calvarial defect model. To induce the mechanical stability, three-dimensional printing was used to produce a stable porous cylinder of polylactide. The cylinder was filled with collagen type I and immobilized BSP and implanted into a femoral defect of critical size in rats. This hybrid material was able to significantly induce bone regeneration. Our study clearly shows the osteogenic effect of BSP when combined with collagen type I as carrier and thereby offers various approaches and options for its use as bioactive molecule in bone substitute materials.
DDC-Sachgruppe:	610 Medizin 610 Medical sciences
Veröffentlichende Institution:	Johannes Gutenberg-Universität Mainz
Organisationseinheit:	FB 04 Medizin
Veröffentlichungsort:	Mainz
ROR:	https://ror.org/023b0x485
DOI:	http://doi.org/10.25358/openscience-8636
Version:	Published version
Publikationstyp:	Zeitschriftenaufsatz
Weitere Angaben zur Dokumentart:	Scientific article
Nutzungsrechte:	CC BY
Informationen zu den Nutzungsrechten:	https://creativecommons.org/licenses/by/4.0/
Zeitschrift:	International journal of bioprinting 8 3
Seitenzahl oder Artikelnummer:	591
Verlag:	Whioce
Verlagsort:	Singapore
Erscheinungsdatum:	2022
ISSN:	2424-8002
DOI der Originalveröffentlichung:	10.18063/ijb.v8i3.591
Enthalten in den Sammlungen:	DFG-491381577-G