Authors:	Kriegel, Anja Schlosser, Christian Habeck, Tanja Dahmen, Christoph Götz, Hermann Clauder, Franziska Armbruster, Franz Paul Baranowski, Andreas Drees, Philipp Rommens, Pol Maria Ritz, Ulrike
Title:	Bone sialoprotein immobilized in collagen type I enhances bone regeneration in vitro and in vivo
Online publication date:	25-Jan-2023
Year of first publication:	2022
Language:	english
Abstract:	The use of bioactive molecules is a promising approach to enhance the bone healing properties of biomaterials. The aim of this study was to define the role of bone sialoprotein (BSP) immobilized in collagen type I in various settings. In vitro studies with human primary osteoblasts in mono- or in co-culture with endothelial cells demonstrated a slightly increased gene expression of osteogenic markers as well as an increased proliferation rate in osteoblasts after application of BSP immobilized in collagen type I. Two critical size bone defect models were used to analyze bone regeneration. BSP incorporated in collagen type I increased bone regeneration only marginally at one concentration in a calvarial defect model. To induce the mechanical stability, three-dimensional printing was used to produce a stable porous cylinder of polylactide. The cylinder was filled with collagen type I and immobilized BSP and implanted into a femoral defect of critical size in rats. This hybrid material was able to significantly induce bone regeneration. Our study clearly shows the osteogenic effect of BSP when combined with collagen type I as carrier and thereby offers various approaches and options for its use as bioactive molecule in bone substitute materials.
DDC:	610 Medizin 610 Medical sciences
Institution:	Johannes Gutenberg-Universität Mainz
Department:	FB 04 Medizin
Place:	Mainz
ROR:	https://ror.org/023b0x485
DOI:	http://doi.org/10.25358/openscience-8636
Version:	Published version
Publication type:	Zeitschriftenaufsatz
Document type specification:	Scientific article
License:	CC BY
Information on rights of use:	https://creativecommons.org/licenses/by/4.0/
Journal:	International journal of bioprinting 8 3
Pages or article number:	591
Publisher:	Whioce
Publisher place:	Singapore
Issue date:	2022
ISSN:	2424-8002
Publisher DOI:	10.18063/ijb.v8i3.591
Appears in collections:	DFG-491381577-G