Gutenberg Open Science: Bone sialoprotein immobilized in collagen type I enhances bone regeneration in vitro and in vivo

Please use this identifier to cite or link to this item: http://doi.org/10.25358/openscience-8636

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DC Field	Value	Language
dc.contributor.author	Kriegel, Anja	-
dc.contributor.author	Schlosser, Christian	-
dc.contributor.author	Habeck, Tanja	-
dc.contributor.author	Dahmen, Christoph	-
dc.contributor.author	Götz, Hermann	-
dc.contributor.author	Clauder, Franziska	-
dc.contributor.author	Armbruster, Franz Paul	-
dc.contributor.author	Baranowski, Andreas	-
dc.contributor.author	Drees, Philipp	-
dc.contributor.author	Rommens, Pol Maria	-
dc.contributor.author	Ritz, Ulrike	-
dc.date.accessioned	2023-01-25T11:01:15Z	-
dc.date.available	2023-01-25T11:01:15Z	-
dc.date.issued	2022	-
dc.identifier.uri	https://openscience.ub.uni-mainz.de/handle/20.500.12030/8652	-
dc.description.abstract	The use of bioactive molecules is a promising approach to enhance the bone healing properties of biomaterials. The aim of this study was to define the role of bone sialoprotein (BSP) immobilized in collagen type I in various settings. In vitro studies with human primary osteoblasts in mono- or in co-culture with endothelial cells demonstrated a slightly increased gene expression of osteogenic markers as well as an increased proliferation rate in osteoblasts after application of BSP immobilized in collagen type I. Two critical size bone defect models were used to analyze bone regeneration. BSP incorporated in collagen type I increased bone regeneration only marginally at one concentration in a calvarial defect model. To induce the mechanical stability, three-dimensional printing was used to produce a stable porous cylinder of polylactide. The cylinder was filled with collagen type I and immobilized BSP and implanted into a femoral defect of critical size in rats. This hybrid material was able to significantly induce bone regeneration. Our study clearly shows the osteogenic effect of BSP when combined with collagen type I as carrier and thereby offers various approaches and options for its use as bioactive molecule in bone substitute materials.	en_GB
dc.description.sponsorship	Gefördert durch die Deutsche Forschungsgemeinschaft (DFG) - Projektnummer 491381577	de
dc.language.iso	eng	de
dc.rights	CC BY	*
dc.rights.uri	https://creativecommons.org/licenses/by/4.0/	*
dc.subject.ddc	610 Medizin	de_DE
dc.subject.ddc	610 Medical sciences	en_GB
dc.title	Bone sialoprotein immobilized in collagen type I enhances bone regeneration in vitro and in vivo	en_GB
dc.type	Zeitschriftenaufsatz	de
dc.identifier.doi	http://doi.org/10.25358/openscience-8636	-
jgu.type.contenttype	Scientific article	de
jgu.type.dinitype	article	en_GB
jgu.type.version	Published version	de
jgu.type.resource	Text	de
jgu.organisation.department	FB 04 Medizin	de
jgu.organisation.number	2700	-
jgu.organisation.name	Johannes Gutenberg-Universität Mainz	-
jgu.rights.accessrights	openAccess	-
jgu.journal.title	International journal of bioprinting	de
jgu.journal.volume	8	de
jgu.journal.issue	3	de
jgu.pages.alternative	591	de
jgu.publisher.year	2022	-
jgu.publisher.name	Whioce	de
jgu.publisher.place	Singapore	de
jgu.publisher.issn	2424-8002	de
jgu.organisation.place	Mainz	-
jgu.subject.ddccode	610	de
jgu.publisher.doi	10.18063/ijb.v8i3.591	de
jgu.organisation.ror	https://ror.org/023b0x485	-
jgu.subject.dfg	Lebenswissenschaften	de
Appears in collections:	DFG-491381577-G

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