Authors:	Müller-Heupt, Lena Katharina Wiesmann, Nadine Schröder, Sofia Korkmaz, Yüksel Vierengel, Nina Groß, Jonathan Dahm, Rolf Deschner, James Opatz, Till Brieger, Juergen Al-Nawas, Bilal Kämmerer, Peer W.
Title:	Extracts of Rheum palmatum and Aloe vera show beneficial properties for the synergistic improvement of oral wound healing
Online publication date:	24-Jan-2023
Year of first publication:	2022
Language:	english
Abstract:	Various local and systemic factors compromise oral wound healing and may lead to wound dehiscence, inflammation, or ulcers. Currently, there is a lack of topical therapeutical options. Thus, this study aimed to investigate the effect of Aloe vera (AV) and Rheum palmatum root (RPR) on oral wound healing capacity in vitro. The effect of AV and RPR on human primary fibroblast viability and migration was studied by measuring metabolic activity and gap closure in a scratch assay. Furthermore, cell cycle distribution and cytoskeletal features were analyzed. Antimicrobial activity against the oral pathogen Porphyromonas gingivalis was evaluated by broth microdilution assay. AV and RPR increased fibroblast migration after single agent treatment. Synergistic effects of the plant extract combination were observed regarding cellular migration which were confirmed by calculation of the phenomenological combination index (pCI), whereas the cell cycle distribution was not influenced. Furthermore, the combination of AV and RPR showed synergistic antibacterial effects as determined by the fractional inhibitory concentration index. This study demonstrated that the combination of AV and RPR can promote the migration of human primary fibroblasts in vitro and exert antimicrobial efficacy against P. gingivalis, suggesting these compounds for the topical treatment of wound healing disorders.
DDC:	610 Medizin 610 Medical sciences
Institution:	Johannes Gutenberg-Universität Mainz
Department:	FB 04 Medizin
Place:	Mainz
ROR:	https://ror.org/023b0x485
DOI:	http://doi.org/10.25358/openscience-8612
Version:	Published version
Publication type:	Zeitschriftenaufsatz
Document type specification:	Scientific article
License:	CC BY
Information on rights of use:	https://creativecommons.org/licenses/by/4.0/
Journal:	Pharmaceutics 14 10
Pages or article number:	2060
Publisher:	MDPI
Publisher place:	Basel
Issue date:	2022
ISSN:	1999-4923
Publisher DOI:	10.3390/pharmaceutics14102060
Appears in collections:	DFG-491381577-G