Please use this identifier to cite or link to this item:
http://doi.org/10.25358/openscience-8611
Authors: | Warm, Davide Bassetti, Davide Schroer, Jonas Luhmann, Heiko J. Sinning, Anne |
Title: | Spontaneous activity predicts survival of developing cortical neurons |
Online publication date: | 24-Jan-2023 |
Year of first publication: | 2022 |
Language: | english |
Abstract: | Spontaneous activity plays a crucial role in brain development by coordinating the integration of immature neurons into emerging cortical networks. High levels and complex patterns of spontaneous activity are generally associated with low rates of apoptosis in the cortex. However, whether spontaneous activity patterns directly encode for survival of individual cortical neurons during development remains an open question. Here, we longitudinally investigated spontaneous activity and apoptosis in developing cortical cultures, combining extracellular electrophysiology with calcium imaging. These experiments demonstrated that the early occurrence of calcium transients was strongly linked to neuronal survival. Silent neurons exhibited a higher probability of cell death, whereas high frequency spiking and burst behavior were almost exclusively detected in surviving neurons. In local neuronal clusters, activity of neighboring neurons exerted a pro-survival effect, whereas on the functional level, networks with a high modular topology were associated with lower cell death rates. Using machine learning algorithms, cell fate of individual neurons was predictable through the integration of spontaneous activity features. Our results indicate that high frequency spiking activity constrains apoptosis in single neurons through sustained calcium rises and thereby consolidates networks in which a high modular topology is reached during early development. |
DDC: | 610 Medizin 610 Medical sciences |
Institution: | Johannes Gutenberg-Universität Mainz |
Department: | FB 04 Medizin |
Place: | Mainz |
ROR: | https://ror.org/023b0x485 |
DOI: | http://doi.org/10.25358/openscience-8611 |
Version: | Published version |
Publication type: | Zeitschriftenaufsatz |
Document type specification: | Scientific article |
License: | CC BY |
Information on rights of use: | https://creativecommons.org/licenses/by/4.0/ |
Journal: | Frontiers in cell and developmental biology 10 |
Pages or article number: | 937761 |
Publisher: | Frontiers Media |
Publisher place: | Lausanne |
Issue date: | 2022 |
ISSN: | 2296-634X |
Publisher DOI: | 10.3389/fcell.2022.937761 |
Appears in collections: | DFG-491381577-G |
Files in This Item:
File | Description | Size | Format | ||
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![]() | spontaneous_activity_predicts-20230119112356188.PDF | 3.38 MB | Adobe PDF | View/Open |