Please use this identifier to cite or link to this item:
http://doi.org/10.25358/openscience-8177
Authors: | Neuberger, Elmo W. I. Sontag, Stephanie Brahmer, Alexandra Philippi, Keito F. A. Radsak, Markus P. Wagner, Wolfgang Simon, Perikles |
Title: | Physical activity specifically evokes release of cell-free DNA from granulocytes thereby affecting liquid biopsy |
Online publication date: | 7-Nov-2022 |
Year of first publication: | 2022 |
Language: | english |
Abstract: | Physical activity impacts immune homeostasis and leads to rapid and marked increase in cell-free DNA (cfDNA). However, the origin of cfDNA during exercise remains elusive and it is unknown if physical activity could improve or interfere with methylation based liquid biopsy. We analyzed the methylation levels of four validated CpGs representing cfDNA from granulocytes, lymphocytes, monocytes, and non-hematopoietic cells, in healthy individuals in response to exercise, and in patients with hematological malignancies under resting conditions. The analysis revealed that physical activity almost exclusively triggered DNA release from granulocytes, highlighting the relevance as a pre-analytical variable which could compromise diagnostic accuracy. |
DDC: | 570 Biowissenschaften 570 Life sciences 610 Medizin 610 Medical sciences 796 Sport 796 Athletic and outdoor sports and games |
Institution: | Johannes Gutenberg-Universität Mainz |
Department: | FB 02 Sozialwiss., Medien u. Sport |
Place: | Mainz |
ROR: | https://ror.org/023b0x485 |
DOI: | http://doi.org/10.25358/openscience-8177 |
Version: | Published version |
Publication type: | Zeitschriftenaufsatz |
Document type specification: | Scientific article |
License: | CC BY |
Information on rights of use: | https://creativecommons.org/licenses/by/4.0/ |
Journal: | Clinical epigenetics 14 |
Pages or article number: | 29 |
Publisher: | Springer Nature |
Publisher place: | London |
Issue date: | 2022 |
ISSN: | 1868-7083 |
Publisher DOI: | 10.1186/s13148-022-01245-3 |
Appears in collections: | DFG-491381577-G |
Files in This Item:
File | Description | Size | Format | ||
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physical_activity_specificall-20221025145207242.pdf | 1.96 MB | Adobe PDF | View/Open |