Authors:	Neuberger, Elmo W. I. Sontag, Stephanie Brahmer, Alexandra Philippi, Keito F. A. Radsak, Markus P. Wagner, Wolfgang Simon, Perikles
Title:	Physical activity specifically evokes release of cell-free DNA from granulocytes thereby affecting liquid biopsy
Online publication date:	7-Nov-2022
Year of first publication:	2022
Language:	english
Abstract:	Physical activity impacts immune homeostasis and leads to rapid and marked increase in cell-free DNA (cfDNA). However, the origin of cfDNA during exercise remains elusive and it is unknown if physical activity could improve or interfere with methylation based liquid biopsy. We analyzed the methylation levels of four validated CpGs representing cfDNA from granulocytes, lymphocytes, monocytes, and non-hematopoietic cells, in healthy individuals in response to exercise, and in patients with hematological malignancies under resting conditions. The analysis revealed that physical activity almost exclusively triggered DNA release from granulocytes, highlighting the relevance as a pre-analytical variable which could compromise diagnostic accuracy.
DDC:	570 Biowissenschaften 570 Life sciences 610 Medizin 610 Medical sciences 796 Sport 796 Athletic and outdoor sports and games
Institution:	Johannes Gutenberg-Universität Mainz
Department:	FB 02 Sozialwiss., Medien u. Sport
Place:	Mainz
ROR:	https://ror.org/023b0x485
DOI:	http://doi.org/10.25358/openscience-8177
Version:	Published version
Publication type:	Zeitschriftenaufsatz
Document type specification:	Scientific article
License:	CC BY
Information on rights of use:	https://creativecommons.org/licenses/by/4.0/
Journal:	Clinical epigenetics 14
Pages or article number:	29
Publisher:	Springer Nature
Publisher place:	London
Issue date:	2022
ISSN:	1868-7083
Publisher DOI:	10.1186/s13148-022-01245-3
Appears in collections:	DFG-491381577-G