Autoren:	Fan, Xinlong Walther, Andreas
Titel:	pH feedback lifecycles programmed by enzymatic logic gates using common foods as fuels
Online-Publikationsdatum:	16-Nov-2022
Erscheinungsdatum:	2021
Sprache des Dokuments:	Englisch
Zusammenfassung/Abstract:	Artificial temporal signaling systems, which mimic living out-of-equilibrium conditions, have made large progress. However, systems programmed by enzymatic reaction networks in multicomponent and unknown environments, and using biocompatible components remain a challenge. Herein, we demonstrate an approach to program temporal pH signals by enzymatic logic gates. They are realized by an enzymatic disaccharide-to-monosaccharide-to-sugar acid reaction cascade catalyzed by two metabolic chains: invertase-glucose oxidase and β-galactosidase-glucose oxidase, respectively. Lifetimes of the transient pH signal can be programmed from less than 15 min to more than 1 day. We study enzymatic kinetics of the reaction cascades and reveal the underlying regulatory mechanisms. Operating with all-food grade chemicals and coupling to self-regulating hydrogel, our system is quite robust to work in a complicated medium with unknown components and in a biocompatible fashion.
DDC-Sachgruppe:	540 Chemie 540 Chemistry and allied sciences
Veröffentlichende Institution:	Johannes Gutenberg-Universität Mainz
Organisationseinheit:	FB 09 Chemie, Pharmazie u. Geowissensch.
Veröffentlichungsort:	Mainz
ROR:	https://ror.org/023b0x485
DOI:	http://doi.org/10.25358/openscience-8151
Version:	Published version
Publikationstyp:	Zeitschriftenaufsatz
Nutzungsrechte:	CC BY-NC-ND
Informationen zu den Nutzungsrechten:	https://creativecommons.org/licenses/by-nc-nd/4.0/
Zeitschrift:	Angewandte Chemie : International edition 60 20
Seitenzahl oder Artikelnummer:	11398 11405
Verlag:	Wiley-VCH
Verlagsort:	Weinheim
Erscheinungsdatum:	2021
ISSN:	1521-3773
DOI der Originalveröffentlichung:	10.1002/anie.202017003
Enthalten in den Sammlungen:	JGU-Publikationen