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Authors: Fan, Xinlong
Walther, Andreas
Title: pH feedback lifecycles programmed by enzymatic logic gates using common foods as fuels
Online publication date: 16-Nov-2022
Year of first publication: 2021
Language: english
Abstract: Artificial temporal signaling systems, which mimic living out-of-equilibrium conditions, have made large progress. However, systems programmed by enzymatic reaction networks in multicomponent and unknown environments, and using biocompatible components remain a challenge. Herein, we demonstrate an approach to program temporal pH signals by enzymatic logic gates. They are realized by an enzymatic disaccharide-to-monosaccharide-to-sugar acid reaction cascade catalyzed by two metabolic chains: invertase-glucose oxidase and β-galactosidase-glucose oxidase, respectively. Lifetimes of the transient pH signal can be programmed from less than 15 min to more than 1 day. We study enzymatic kinetics of the reaction cascades and reveal the underlying regulatory mechanisms. Operating with all-food grade chemicals and coupling to self-regulating hydrogel, our system is quite robust to work in a complicated medium with unknown components and in a biocompatible fashion.
DDC: 540 Chemie
540 Chemistry and allied sciences
Institution: Johannes Gutenberg-Universität Mainz
Department: FB 09 Chemie, Pharmazie u. Geowissensch.
Place: Mainz
Version: Published version
Publication type: Zeitschriftenaufsatz
License: CC BY-NC-ND
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Journal: Angewandte Chemie : International edition
Pages or article number: 11398
Publisher: Wiley-VCH
Publisher place: Weinheim
Issue date: 2021
ISSN: 1521-3773
Publisher DOI: 10.1002/anie.202017003
Appears in collections:JGU-Publikationen

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