Gutenberg Open Science: C4-dicarboxylates and L-aspartate utilization by Escherichia coli K-12 in the mouse intestine : L-aspartate as a major substrate for fumarate respiration and as a nitrogen source

Please use this identifier to cite or link to this item: http://doi.org/10.25358/openscience-8147

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DC Field	Value	Language
dc.contributor.author	Schubert, Christopher	-
dc.contributor.author	Winter, Maria	-
dc.contributor.author	Ebert-Jung, Andrea	-
dc.contributor.author	Kierszniowska, Sylwia	-
dc.contributor.author	Nagel-Wolfrum, Kerstin	-
dc.contributor.author	Schramm, Thorben	-
dc.contributor.author	Link, Hannes	-
dc.contributor.author	Winter, Sebastian	-
dc.contributor.author	Unden, Gottfried	-
dc.date.accessioned	2022-11-15T11:08:50Z	-
dc.date.available	2022-11-15T11:08:50Z	-
dc.date.issued	2021	-
dc.identifier.uri	https://openscience.ub.uni-mainz.de/handle/20.500.12030/8162	-
dc.description.abstract	C4-dicarboxylates, such as fumarate, l-malate and l-aspartate represent substrates for anaerobic growth of Escherichia coli by fumarate respiration. Here, we determined whether C4-dicarboxylate metabolism, as well as fumarate respiration, contribute to colonization of the mammalian intestinal tract. Metabolite profiling revealed that the murine small intestine contained high and low levels of l-aspartate and l-malate respectively, whereas fumarate was nearly absent. Under laboratory conditions, addition of C4-dicarboxylate at concentrations corresponding to the levels of the C4-dicarboxylates in the small intestine (2.6 mmol kg−1 dry weight) induced the dcuBp-lacZ reporter gene (67% of maximal) in a DcuS-DcuR-dependent manner. In addition to its role as a precursor for fumarate respiration, l-aspartate was able to supply all the nitrogen required for anaerobically growing E. coli. DcuS-DcuR-dependent genes were transcribed in the murine intestine, and mutants with defective anaerobic C4-dicarboxylate metabolism (dcuSR, frdA, dcuB, dcuA and aspA genes) were impaired for colonizing the murine gut. We conclude that l-aspartate plays an important role in providing fumarate for fumarate respiration and supplying nitrogen for E. coli in the mouse intestine.	en_GB
dc.language.iso	eng	de
dc.rights	CC BY	*
dc.rights.uri	https://creativecommons.org/licenses/by/4.0/	*
dc.subject.ddc	570 Biowissenschaften	de_DE
dc.subject.ddc	570 Life sciences	en_GB
dc.title	C4-dicarboxylates and L-aspartate utilization by Escherichia coli K-12 in the mouse intestine : L-aspartate as a major substrate for fumarate respiration and as a nitrogen source	en_GB
dc.type	Zeitschriftenaufsatz	de
dc.identifier.doi	http://doi.org/10.25358/openscience-8147	-
jgu.type.dinitype	article	en_GB
jgu.type.version	Published version	de
jgu.type.resource	Text	de
jgu.organisation.department	FB 10 Biologie	de
jgu.organisation.number	7970	-
jgu.organisation.name	Johannes Gutenberg-Universität Mainz	-
jgu.rights.accessrights	openAccess	-
jgu.journal.title	Environmental microbiology	de
jgu.journal.volume	23	de
jgu.journal.issue	5	de
jgu.pages.start	2564	de
jgu.pages.end	2577	de
jgu.publisher.year	2021	-
jgu.publisher.name	Wiley-Blackwell	de
jgu.publisher.place	Oxford u.a.	de
jgu.publisher.issn	1462-2920	de
jgu.organisation.place	Mainz	-
jgu.subject.ddccode	570	de
jgu.publisher.doi	10.1111/1462-2920.15478	de
jgu.organisation.ror	https://ror.org/023b0x485	-
Appears in collections:	JGU-Publikationen

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