Please use this identifier to cite or link to this item:
http://doi.org/10.25358/openscience-8147
Authors: | Schubert, Christopher Winter, Maria Ebert-Jung, Andrea Kierszniowska, Sylwia Nagel-Wolfrum, Kerstin Schramm, Thorben Link, Hannes Winter, Sebastian Unden, Gottfried |
Title: | C4-dicarboxylates and L-aspartate utilization by Escherichia coli K-12 in the mouse intestine : L-aspartate as a major substrate for fumarate respiration and as a nitrogen source |
Online publication date: | 15-Nov-2022 |
Year of first publication: | 2021 |
Language: | english |
Abstract: | C4-dicarboxylates, such as fumarate, l-malate and l-aspartate represent substrates for anaerobic growth of Escherichia coli by fumarate respiration. Here, we determined whether C4-dicarboxylate metabolism, as well as fumarate respiration, contribute to colonization of the mammalian intestinal tract. Metabolite profiling revealed that the murine small intestine contained high and low levels of l-aspartate and l-malate respectively, whereas fumarate was nearly absent. Under laboratory conditions, addition of C4-dicarboxylate at concentrations corresponding to the levels of the C4-dicarboxylates in the small intestine (2.6 mmol kg−1 dry weight) induced the dcuBp-lacZ reporter gene (67% of maximal) in a DcuS-DcuR-dependent manner. In addition to its role as a precursor for fumarate respiration, l-aspartate was able to supply all the nitrogen required for anaerobically growing E. coli. DcuS-DcuR-dependent genes were transcribed in the murine intestine, and mutants with defective anaerobic C4-dicarboxylate metabolism (dcuSR, frdA, dcuB, dcuA and aspA genes) were impaired for colonizing the murine gut. We conclude that l-aspartate plays an important role in providing fumarate for fumarate respiration and supplying nitrogen for E. coli in the mouse intestine. |
DDC: | 570 Biowissenschaften 570 Life sciences |
Institution: | Johannes Gutenberg-Universität Mainz |
Department: | FB 10 Biologie |
Place: | Mainz |
ROR: | https://ror.org/023b0x485 |
DOI: | http://doi.org/10.25358/openscience-8147 |
Version: | Published version |
Publication type: | Zeitschriftenaufsatz |
License: | CC BY |
Information on rights of use: | https://creativecommons.org/licenses/by/4.0/ |
Journal: | Environmental microbiology 23 5 |
Pages or article number: | 2564 2577 |
Publisher: | Wiley-Blackwell |
Publisher place: | Oxford u.a. |
Issue date: | 2021 |
ISSN: | 1462-2920 |
Publisher DOI: | 10.1111/1462-2920.15478 |
Appears in collections: | JGU-Publikationen |
Files in This Item:
File | Description | Size | Format | ||
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![]() | c4dicarboxylates_and_lasparta-20221021113721677.pdf | 1 MB | Adobe PDF | View/Open |