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http://doi.org/10.25358/openscience-8147
Autoren: | Schubert, Christopher Winter, Maria Ebert-Jung, Andrea Kierszniowska, Sylwia Nagel-Wolfrum, Kerstin Schramm, Thorben Link, Hannes Winter, Sebastian Unden, Gottfried |
Titel: | C4-dicarboxylates and L-aspartate utilization by Escherichia coli K-12 in the mouse intestine : L-aspartate as a major substrate for fumarate respiration and as a nitrogen source |
Online-Publikationsdatum: | 15-Nov-2022 |
Erscheinungsdatum: | 2021 |
Sprache des Dokuments: | Englisch |
Zusammenfassung/Abstract: | C4-dicarboxylates, such as fumarate, l-malate and l-aspartate represent substrates for anaerobic growth of Escherichia coli by fumarate respiration. Here, we determined whether C4-dicarboxylate metabolism, as well as fumarate respiration, contribute to colonization of the mammalian intestinal tract. Metabolite profiling revealed that the murine small intestine contained high and low levels of l-aspartate and l-malate respectively, whereas fumarate was nearly absent. Under laboratory conditions, addition of C4-dicarboxylate at concentrations corresponding to the levels of the C4-dicarboxylates in the small intestine (2.6 mmol kg−1 dry weight) induced the dcuBp-lacZ reporter gene (67% of maximal) in a DcuS-DcuR-dependent manner. In addition to its role as a precursor for fumarate respiration, l-aspartate was able to supply all the nitrogen required for anaerobically growing E. coli. DcuS-DcuR-dependent genes were transcribed in the murine intestine, and mutants with defective anaerobic C4-dicarboxylate metabolism (dcuSR, frdA, dcuB, dcuA and aspA genes) were impaired for colonizing the murine gut. We conclude that l-aspartate plays an important role in providing fumarate for fumarate respiration and supplying nitrogen for E. coli in the mouse intestine. |
DDC-Sachgruppe: | 570 Biowissenschaften 570 Life sciences |
Veröffentlichende Institution: | Johannes Gutenberg-Universität Mainz |
Organisationseinheit: | FB 10 Biologie |
Veröffentlichungsort: | Mainz |
ROR: | https://ror.org/023b0x485 |
DOI: | http://doi.org/10.25358/openscience-8147 |
Version: | Published version |
Publikationstyp: | Zeitschriftenaufsatz |
Nutzungsrechte: | CC BY |
Informationen zu den Nutzungsrechten: | https://creativecommons.org/licenses/by/4.0/ |
Zeitschrift: | Environmental microbiology 23 5 |
Seitenzahl oder Artikelnummer: | 2564 2577 |
Verlag: | Wiley-Blackwell |
Verlagsort: | Oxford u.a. |
Erscheinungsdatum: | 2021 |
ISSN: | 1462-2920 |
DOI der Originalveröffentlichung: | 10.1111/1462-2920.15478 |
Enthalten in den Sammlungen: | JGU-Publikationen |
Dateien zu dieser Ressource:
Datei | Beschreibung | Größe | Format | ||
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c4dicarboxylates_and_lasparta-20221021113721677.pdf | 1 MB | Adobe PDF | Öffnen/Anzeigen |