Gutenberg Open Science: A barcoding pipeline for mosquito surveillance in Nepal, a biodiverse dengue-endemic country

Please use this identifier to cite or link to this item: http://doi.org/10.25358/openscience-8103

Full metadata record

DC Field	Value	Language
dc.contributor.author	Hartke, Juliane	-
dc.contributor.author	Reuss, Friederike	-
dc.contributor.author	Kramer, Isabelle Marie	-
dc.contributor.author	Magdeburg, Axel	-
dc.contributor.author	Deblauwe, Isra	-
dc.contributor.author	Tuladhar, Reshma	-
dc.contributor.author	Gautam, Ishan	-
dc.contributor.author	Dhimal, Meghnath	-
dc.contributor.author	Müller, Ruth	-
dc.date.accessioned	2022-11-07T09:11:37Z	-
dc.date.available	2022-11-07T09:11:37Z	-
dc.date.issued	2022	-
dc.identifier.uri	https://openscience.ub.uni-mainz.de/handle/20.500.12030/8118	-
dc.description.abstract	Background Vector-borne diseases are on the rise on a global scale, which is anticipated to further accelerate because of anthropogenic climate change. Resource-limited regions are especially hard hit by this increment with the currently implemented surveillance programs being inadequate for the observed expansion of potential vector species. Cost-effective methods that can be easily implemented in resource-limited settings, e.g. under field conditions, are thus urgently needed to function as an early warning system for vector-borne disease epidemics. Our aim was to enhance entomological capacity in Nepal, a country with endemicity of numerous vector-borne diseases and with frequent outbreaks of dengue fever. Methods We used a field barcoding pipeline based on DNA nanopore sequencing (Oxford Nanopore Technologies) and verified its use for different mosquito life stages and storage methods. We furthermore hosted an online workshop to facilitate knowledge transfer to Nepalese scientific experts from different disciplines. Results The use of the barcoding pipeline could be verified for adult mosquitos and eggs, as well as for homogenized samples, dried specimens, samples that were stored in ethanol and frozen tissue. The transfer of knowledge was successful, as reflected by feedback from the participants and their wish to implement the method. Conclusions Cost effective strategies are urgently needed to assess the likelihood of disease outbreaks. We were able to show that field sequencing provides a solution that is cost-effective, undemanding in its implementation and easy to learn. The knowledge transfer to Nepalese scientific experts from different disciplines provides an opportunity for sustainable implementation of low-cost portable sequencing solutions in Nepal.	en_GB
dc.description.sponsorship	Gefördert durch die Deutsche Forschungsgemeinschaft (DFG) - Projektnummer 491381577	de
dc.language.iso	eng	de
dc.rights	CC BY	*
dc.rights.uri	https://creativecommons.org/licenses/by/4.0/	*
dc.subject.ddc	570 Biowissenschaften	de_DE
dc.subject.ddc	570 Life sciences	en_GB
dc.title	A barcoding pipeline for mosquito surveillance in Nepal, a biodiverse dengue-endemic country	en_GB
dc.type	Zeitschriftenaufsatz	de
dc.identifier.doi	http://doi.org/10.25358/openscience-8103	-
jgu.type.contenttype	Scientific article	de
jgu.type.dinitype	article	en_GB
jgu.type.version	Published version	de
jgu.type.resource	Text	de
jgu.organisation.department	FB 10 Biologie	de
jgu.organisation.number	7970	-
jgu.organisation.name	Johannes Gutenberg-Universität Mainz	-
jgu.rights.accessrights	openAccess	-
jgu.journal.title	Parasites & vectors	de
jgu.journal.volume	15	de
jgu.pages.alternative	145	de
jgu.publisher.year	2022	-
jgu.publisher.name	BioMed Central	de
jgu.publisher.place	London	de
jgu.publisher.issn	1756-3305	de
jgu.organisation.place	Mainz	-
jgu.subject.ddccode	570	de
jgu.publisher.doi	10.1186/s13071-022-05255-1	de
jgu.organisation.ror	https://ror.org/023b0x485	-
jgu.subject.dfg	Naturwissenschaften	de
Appears in collections:	DFG-491381577-G

Files in This Item:

File	Description	Size	Format
a_barcoding_pipeline_for_mosq-20221019114639088.pdf	Article	1.23 MB	Adobe PDF	View/Open
a_barcoding_pipeline_for_mosq-20221019114648734.docx	Additional file 1	811.47 kB	Microsoft Word XML	View/Open
a_barcoding_pipeline_for_mosq-20221019114721221.pdf	Additional file 2	1.84 MB	Adobe PDF	View/Open
a_barcoding_pipeline_for_mosq-20221019114741332.pdf	Additional file 3	1.29 MB	Adobe PDF	View/Open
a_barcoding_pipeline_for_mosq-20221019114749248.pdf	Additional file 4	2.92 MB	Adobe PDF	View/Open

Show simple item record