Authors:	Motorin, Yuri Helm, Mark
Title:	RNA nucleotide methylation: 2021 update
Online publication date:	8-Nov-2022
Year of first publication:	2022
Language:	english
Abstract:	Among RNA modifications, transfer of methylgroups from the typical cofactor S-adenosyl-l-methionine by methyltransferases (MTases) to RNA is by far the most common reaction. Since our last review about a decade ago, the field has witnessed the re-emergence of mRNA methylation as an important mechanism in gene regulation. Attention has then spread to many other RNA species; all being included into the newly coined concept of the “epitranscriptome.” The focus moved from prokaryotes and single cell eukaryotes as model organisms to higher eukaryotes, in particular to mammals. The perception of the field has dramatically changed over the past decade. A previous lack of phenotypes in knockouts in single cell organisms has been replaced by the apparition of MTases in numerous disease models and clinical investigations. Major driving forces of the field include methylation mapping techniques, as well as the characterization of the various MTases, termed “writers.” The latter term has spilled over from DNA modification in the neighboring epigenetics field, along with the designations “readers,” applied to mediators of biological effects upon specific binding to a methylated RNA. Furthermore “eraser” enzymes effect the newly discovered oxidative removal of methylgroups. A sense of reversibility and dynamics has replaced the older perception of RNA modification as a concrete-cast, irreversible part of RNA maturation. A related concept concerns incompletely methylated residues, which, through permutation of each site, lead to inhomogeneous populations of numerous modivariants. This review recapitulates the major developments of the past decade outlined above, and attempts a prediction of upcoming trends.
DDC:	540 Chemie 540 Chemistry and allied sciences 610 Medizin 610 Medical sciences
Institution:	Johannes Gutenberg-Universität Mainz
Department:	FB 09 Chemie, Pharmazie u. Geowissensch.
Place:	Mainz
ROR:	https://ror.org/023b0x485
DOI:	http://doi.org/10.25358/openscience-8035
Version:	Published version
Publication type:	Zeitschriftenaufsatz
License:	CC BY
Information on rights of use:	https://creativecommons.org/licenses/by/4.0/
Journal:	Wiley interdisciplinary reviews : RNA 13 1
Pages or article number:	e1691
Publisher:	Wiley-Blackwell
Publisher place:	Malden, MA
Issue date:	2022
ISSN:	1757-7012
Publisher DOI:	10.1002/wrna.1691
Appears in collections:	JGU-Publikationen