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http://doi.org/10.25358/openscience-802
Autoren: | Breuksch, Ines Welter, Jonas Bauer, Heide-Katharina Enklaar, Thorsten Frees, Sebastian Thüroff, Joachim W. Hasenburg, Annette Prawitt, Dirk Brenner, Walburgis |
Titel: | In renal cell carcinoma the PTEN splice variant PTEN-Δ shows similar function as the tumor suppressor PTEN itself |
Online-Publikationsdatum: | 6-Aug-2018 |
Erscheinungsdatum: | 2018 |
Sprache des Dokuments: | Englisch |
Zusammenfassung/Abstract: | BACKGROUND: Loss of PTEN is involved in tumor progression of several tumor entities including renal cell carcinoma (RCC). During the translation process PTEN generates a number of splice variants, including PTEN-Δ. We analyzed the impact of PTEN-Δ in RCC progression. METHODS: In specimens of RCC patients the expression of PTEN-Δ and PTEN was quantified. The PTEN expressing RCC cell line A498 and the PTEN deficient 786-O cell line were stably transfected with the PTEN-Δ or PTEN transcript. In Caki-1 cells that highly express PTEN-Δ, this isoform was knocked down by siRNA. Cell migration, adhesion, apoptosis and signaling pathways activities were consequently analyzed in vitro. RESULTS: Patients with a higher PTEN-Δ expression had a longer lymph node metastasis free and overall survival. In RCC specimens, the PTEN-Δ expression correlated with the PTEN expression. PTEN-Δ as well as PTEN induced a reduced migration when using extracellular matrix (ECM) compounds as chemotaxins. This effect was confirmed by knockdown of PTEN-Δ, inducing an enhanced migration. Likewise a decreased adhesion on these ECM components could be shown in PTEN-Δ and PTEN transfected cells. The apoptosis rate was slightly increased by PTEN-Δ. In a phospho-kinase array and Western blot analyses a consequently reduced activity of AKT, p38 and JNK could be shown. CONCLUSIONS: We could show that the PTEN splice variant PTEN-Δ acts similar to PTEN in a tumor suppressive manner, suggesting synergistic effects of the two isoforms. The impact of PTEN-Δ in context of tumor progression should thus be taken into account when generating new therapeutic options targeting PTEN signaling in RCC. |
DDC-Sachgruppe: | 610 Medizin 610 Medical sciences |
Veröffentlichende Institution: | Johannes Gutenberg-Universität Mainz |
Organisationseinheit: | FB 04 Medizin |
Veröffentlichungsort: | Mainz |
ROR: | https://ror.org/023b0x485 |
DOI: | http://doi.org/10.25358/openscience-802 |
URN: | urn:nbn:de:hebis:77-publ-584125 |
Version: | Published version |
Publikationstyp: | Zeitschriftenaufsatz |
Nutzungsrechte: | CC BY |
Informationen zu den Nutzungsrechten: | https://creativecommons.org/licenses/by/4.0/ |
Zeitschrift: | Cell communication and signaling 16 |
Seitenzahl oder Artikelnummer: | Art. 35 |
Verlag: | BioMed Central |
Verlagsort: | London |
Erscheinungsdatum: | 2018 |
ISSN: | 1478-811X |
URL der Originalveröffentlichung: | http://dx.doi.org/10.1186/s12964-018-0247-9 |
DOI der Originalveröffentlichung: | 10.1186/s12964-018-0247-9 |
Enthalten in den Sammlungen: | JGU-Publikationen |
Dateien zu dieser Ressource:
Datei | Beschreibung | Größe | Format | ||
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58412.pdf | 1.82 MB | Adobe PDF | Öffnen/Anzeigen |