Please use this identifier to cite or link to this item:
http://doi.org/10.25358/openscience-802
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DC Field | Value | Language |
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dc.contributor.author | Breuksch, Ines | - |
dc.contributor.author | Welter, Jonas | - |
dc.contributor.author | Bauer, Heide-Katharina | - |
dc.contributor.author | Enklaar, Thorsten | - |
dc.contributor.author | Frees, Sebastian | - |
dc.contributor.author | Thüroff, Joachim W. | - |
dc.contributor.author | Hasenburg, Annette | - |
dc.contributor.author | Prawitt, Dirk | - |
dc.contributor.author | Brenner, Walburgis | - |
dc.date.accessioned | 2018-08-06T13:24:05Z | - |
dc.date.available | 2018-08-06T15:24:05Z | - |
dc.date.issued | 2018 | - |
dc.identifier.uri | https://openscience.ub.uni-mainz.de/handle/20.500.12030/804 | - |
dc.description.abstract | BACKGROUND: Loss of PTEN is involved in tumor progression of several tumor entities including renal cell carcinoma (RCC). During the translation process PTEN generates a number of splice variants, including PTEN-Δ. We analyzed the impact of PTEN-Δ in RCC progression. METHODS: In specimens of RCC patients the expression of PTEN-Δ and PTEN was quantified. The PTEN expressing RCC cell line A498 and the PTEN deficient 786-O cell line were stably transfected with the PTEN-Δ or PTEN transcript. In Caki-1 cells that highly express PTEN-Δ, this isoform was knocked down by siRNA. Cell migration, adhesion, apoptosis and signaling pathways activities were consequently analyzed in vitro. RESULTS: Patients with a higher PTEN-Δ expression had a longer lymph node metastasis free and overall survival. In RCC specimens, the PTEN-Δ expression correlated with the PTEN expression. PTEN-Δ as well as PTEN induced a reduced migration when using extracellular matrix (ECM) compounds as chemotaxins. This effect was confirmed by knockdown of PTEN-Δ, inducing an enhanced migration. Likewise a decreased adhesion on these ECM components could be shown in PTEN-Δ and PTEN transfected cells. The apoptosis rate was slightly increased by PTEN-Δ. In a phospho-kinase array and Western blot analyses a consequently reduced activity of AKT, p38 and JNK could be shown. CONCLUSIONS: We could show that the PTEN splice variant PTEN-Δ acts similar to PTEN in a tumor suppressive manner, suggesting synergistic effects of the two isoforms. The impact of PTEN-Δ in context of tumor progression should thus be taken into account when generating new therapeutic options targeting PTEN signaling in RCC. | en_GB |
dc.language.iso | eng | - |
dc.rights | CC BY | de_DE |
dc.rights.uri | https://creativecommons.org/licenses/by/4.0/ | - |
dc.subject.ddc | 610 Medizin | de_DE |
dc.subject.ddc | 610 Medical sciences | en_GB |
dc.title | In renal cell carcinoma the PTEN splice variant PTEN-Δ shows similar function as the tumor suppressor PTEN itself | en_GB |
dc.type | Zeitschriftenaufsatz | de_DE |
dc.identifier.urn | urn:nbn:de:hebis:77-publ-584125 | - |
dc.identifier.doi | http://doi.org/10.25358/openscience-802 | - |
jgu.type.dinitype | article | - |
jgu.type.version | Published version | en_GB |
jgu.type.resource | Text | - |
jgu.organisation.department | FB 04 Medizin | - |
jgu.organisation.number | 2700 | - |
jgu.organisation.name | Johannes Gutenberg-Universität Mainz | - |
jgu.rights.accessrights | openAccess | - |
jgu.journal.title | Cell communication and signaling | - |
jgu.journal.volume | 16 | - |
jgu.pages.alternative | Art. 35 | - |
jgu.publisher.year | 2018 | - |
jgu.publisher.name | BioMed Central | - |
jgu.publisher.place | London | - |
jgu.publisher.uri | http://dx.doi.org/10.1186/s12964-018-0247-9 | - |
jgu.publisher.issn | 1478-811X | - |
jgu.organisation.place | Mainz | - |
jgu.subject.ddccode | 610 | - |
opus.date.accessioned | 2018-08-06T13:24:05Z | - |
opus.date.modified | 2018-09-03T07:53:31Z | - |
opus.date.available | 2018-08-06T15:24:05 | - |
opus.subject.dfgcode | 04-205 | - |
opus.organisation.string | FB 04: Medizin: Kinderklinik und Kinderpoliklinik | de_DE |
opus.organisation.string | FB 04: Medizin: Urologische Klinik und Poliklinik | de_DE |
opus.organisation.string | FB 04: Medizin: Klinik und Poliklinik für Geburtshilfe und Frauenkrankheiten | de_DE |
opus.identifier.opusid | 58412 | - |
opus.institute.number | 0429 | - |
opus.institute.number | 0444 | - |
opus.institute.number | 0445 | - |
opus.metadataonly | false | - |
opus.type.contenttype | Keine | de_DE |
opus.type.contenttype | None | en_GB |
opus.affiliated | Frees, Sebastian | - |
opus.affiliated | Thüroff, Joachim W. | - |
opus.affiliated | Hasenburg, Annette | - |
opus.affiliated | Prawitt, Dirk | - |
opus.affiliated | Brenner, Walburgis | - |
jgu.publisher.doi | 10.1186/s12964-018-0247-9 | |
jgu.organisation.ror | https://ror.org/023b0x485 | |
Appears in collections: | JGU-Publikationen |