Please use this identifier to cite or link to this item: http://doi.org/10.25358/openscience-802
Full metadata record
DC FieldValueLanguage
dc.contributor.authorBreuksch, Ines-
dc.contributor.authorWelter, Jonas-
dc.contributor.authorBauer, Heide-Katharina-
dc.contributor.authorEnklaar, Thorsten-
dc.contributor.authorFrees, Sebastian-
dc.contributor.authorThüroff, Joachim W.-
dc.contributor.authorHasenburg, Annette-
dc.contributor.authorPrawitt, Dirk-
dc.contributor.authorBrenner, Walburgis-
dc.date.accessioned2018-08-06T13:24:05Z-
dc.date.available2018-08-06T15:24:05Z-
dc.date.issued2018-
dc.identifier.urihttps://openscience.ub.uni-mainz.de/handle/20.500.12030/804-
dc.description.abstractBACKGROUND: Loss of PTEN is involved in tumor progression of several tumor entities including renal cell carcinoma (RCC). During the translation process PTEN generates a number of splice variants, including PTEN-Δ. We analyzed the impact of PTEN-Δ in RCC progression. METHODS: In specimens of RCC patients the expression of PTEN-Δ and PTEN was quantified. The PTEN expressing RCC cell line A498 and the PTEN deficient 786-O cell line were stably transfected with the PTEN-Δ or PTEN transcript. In Caki-1 cells that highly express PTEN-Δ, this isoform was knocked down by siRNA. Cell migration, adhesion, apoptosis and signaling pathways activities were consequently analyzed in vitro. RESULTS: Patients with a higher PTEN-Δ expression had a longer lymph node metastasis free and overall survival. In RCC specimens, the PTEN-Δ expression correlated with the PTEN expression. PTEN-Δ as well as PTEN induced a reduced migration when using extracellular matrix (ECM) compounds as chemotaxins. This effect was confirmed by knockdown of PTEN-Δ, inducing an enhanced migration. Likewise a decreased adhesion on these ECM components could be shown in PTEN-Δ and PTEN transfected cells. The apoptosis rate was slightly increased by PTEN-Δ. In a phospho-kinase array and Western blot analyses a consequently reduced activity of AKT, p38 and JNK could be shown. CONCLUSIONS: We could show that the PTEN splice variant PTEN-Δ acts similar to PTEN in a tumor suppressive manner, suggesting synergistic effects of the two isoforms. The impact of PTEN-Δ in context of tumor progression should thus be taken into account when generating new therapeutic options targeting PTEN signaling in RCC.en_GB
dc.language.isoeng-
dc.rightsCC BYde_DE
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/-
dc.subject.ddc610 Medizinde_DE
dc.subject.ddc610 Medical sciencesen_GB
dc.titleIn renal cell carcinoma the PTEN splice variant PTEN-Δ shows similar function as the tumor suppressor PTEN itselfen_GB
dc.typeZeitschriftenaufsatzde_DE
dc.identifier.urnurn:nbn:de:hebis:77-publ-584125-
dc.identifier.doihttp://doi.org/10.25358/openscience-802-
jgu.type.dinitypearticle-
jgu.type.versionPublished versionen_GB
jgu.type.resourceText-
jgu.organisation.departmentFB 04 Medizin-
jgu.organisation.number2700-
jgu.organisation.nameJohannes Gutenberg-Universität Mainz-
jgu.rights.accessrightsopenAccess-
jgu.journal.titleCell communication and signaling-
jgu.journal.volume16-
jgu.pages.alternativeArt. 35-
jgu.publisher.year2018-
jgu.publisher.nameBioMed Central-
jgu.publisher.placeLondon-
jgu.publisher.urihttp://dx.doi.org/10.1186/s12964-018-0247-9-
jgu.publisher.issn1478-811X-
jgu.organisation.placeMainz-
jgu.subject.ddccode610-
opus.date.accessioned2018-08-06T13:24:05Z-
opus.date.modified2018-09-03T07:53:31Z-
opus.date.available2018-08-06T15:24:05-
opus.subject.dfgcode04-205-
opus.organisation.stringFB 04: Medizin: Kinderklinik und Kinderpoliklinikde_DE
opus.organisation.stringFB 04: Medizin: Urologische Klinik und Poliklinikde_DE
opus.organisation.stringFB 04: Medizin: Klinik und Poliklinik für Geburtshilfe und Frauenkrankheitende_DE
opus.identifier.opusid58412-
opus.institute.number0429-
opus.institute.number0444-
opus.institute.number0445-
opus.metadataonlyfalse-
opus.type.contenttypeKeinede_DE
opus.type.contenttypeNoneen_GB
opus.affiliatedFrees, Sebastian-
opus.affiliatedThüroff, Joachim W.-
opus.affiliatedHasenburg, Annette-
opus.affiliatedPrawitt, Dirk-
opus.affiliatedBrenner, Walburgis-
jgu.publisher.doi10.1186/s12964-018-0247-9
jgu.organisation.rorhttps://ror.org/023b0x485
Appears in collections:JGU-Publikationen

Files in This Item:
  File Description SizeFormat
Thumbnail
58412.pdf1.82 MBAdobe PDFView/Open