Autoren:	Weckmann, Katja Diefenthäler, Philip Baeken, Marius W. Yusifli, Kamran Turck, Christoph W. Asara, John M. Behl, Christian Hajieva, Parvana
Titel:	Metabolomics profiling reveals differential adaptation of major energy metabolism pathways associated with autophagy upon oxygen and glucose reduction
Online-Publikationsdatum:	2-Aug-2018
Erscheinungsdatum:	2018
Sprache des Dokuments:	Englisch
Zusammenfassung/Abstract:	The ability of cells to rearrange their metabolism plays an important role in compensating the energy shortage and may provide cell survival. Our study focuses on identifing the important adaptational changes under the conditions of oxygen and glucose reduction. Employing mass spectrometry-based metabolomics in combination with biochemistry and microscopy techniques we identified metabolites, proteins and biomolecular pathways alterations in primary human IMR90 fibroblasts upon energy deficits. Multivariate statistical analyses revealed significant treatment specific metabolite level and ratio alterations as well as major energy metabolism pathways like ‘glycolysis’, ‘pentose phosphate pathway’, ‘mitochondrial electron transport chain’ and ‘protein biosynthesis (amino acids)’ indicating an activation of catabolism and reduction of anabolism as important mechanisms of adaptation towards a bioenergetic demand. A treatment-specific induction of the autophagic and mitophagic degradation activity upon oxygen reduction, glucose reduction as well as oxygen-glucose reduction further supports our results. Therefore, we suggest that the observed alterations represent an adaptive response in order to compensate for the cells’ bioenergetics needs that ultimately provide cell survival.
DDC-Sachgruppe:	610 Medizin 610 Medical sciences
Veröffentlichende Institution:	Johannes Gutenberg-Universität Mainz
Organisationseinheit:	FB 04 Medizin
Veröffentlichungsort:	Mainz
ROR:	https://ror.org/023b0x485
DOI:	http://doi.org/10.25358/openscience-798
URN:	urn:nbn:de:hebis:77-publ-584072
Version:	Published version
Publikationstyp:	Zeitschriftenaufsatz
Nutzungsrechte:	CC BY
Informationen zu den Nutzungsrechten:	https://creativecommons.org/licenses/by/4.0/
Zeitschrift:	Scientific reports 8
Seitenzahl oder Artikelnummer:	Art. 2337
Verlag:	Macmillan Publishers Limited, part of Springer Nature
Verlagsort:	London
Erscheinungsdatum:	2018
ISSN:	2045-2322
URL der Originalveröffentlichung:	http://dx.doi.org/10.1038/s41598-018-19421-y
DOI der Originalveröffentlichung:	10.1038/s41598-018-19421-y
Enthalten in den Sammlungen:	JGU-Publikationen