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Authors: Weckmann, Katja
Diefenthäler, Philip
Baeken, Marius W.
Yusifli, Kamran
Turck, Christoph W.
Asara, John M.
Behl, Christian
Hajieva, Parvana
Title: Metabolomics profiling reveals differential adaptation of major energy metabolism pathways associated with autophagy upon oxygen and glucose reduction
Online publication date: 2-Aug-2018
Year of first publication: 2018
Language: english
Abstract: The ability of cells to rearrange their metabolism plays an important role in compensating the energy shortage and may provide cell survival. Our study focuses on identifing the important adaptational changes under the conditions of oxygen and glucose reduction. Employing mass spectrometry-based metabolomics in combination with biochemistry and microscopy techniques we identified metabolites, proteins and biomolecular pathways alterations in primary human IMR90 fibroblasts upon energy deficits. Multivariate statistical analyses revealed significant treatment specific metabolite level and ratio alterations as well as major energy metabolism pathways like ‘glycolysis’, ‘pentose phosphate pathway’, ‘mitochondrial electron transport chain’ and ‘protein biosynthesis (amino acids)’ indicating an activation of catabolism and reduction of anabolism as important mechanisms of adaptation towards a bioenergetic demand. A treatment-specific induction of the autophagic and mitophagic degradation activity upon oxygen reduction, glucose reduction as well as oxygen-glucose reduction further supports our results. Therefore, we suggest that the observed alterations represent an adaptive response in order to compensate for the cells’ bioenergetics needs that ultimately provide cell survival.
DDC: 610 Medizin
610 Medical sciences
Institution: Johannes Gutenberg-Universität Mainz
Department: FB 04 Medizin
Place: Mainz
URN: urn:nbn:de:hebis:77-publ-584072
Version: Published version
Publication type: Zeitschriftenaufsatz
License: CC BY
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Journal: Scientific reports
Pages or article number: Art. 2337
Publisher: Macmillan Publishers Limited, part of Springer Nature
Publisher place: London
Issue date: 2018
ISSN: 2045-2322
Publisher URL:
Publisher DOI: 10.1038/s41598-018-19421-y
Appears in collections:JGU-Publikationen

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