Autoren:	Wilding, Corina Bell, Katharina Funke, S. Beck, Sabine Pfeiffer, Norbert Grus, Franz-Hermann
Titel:	GFAP antibodies show protective effect on oxidatively stressed neuroretinal cells via interaction with ERP57
Online-Publikationsdatum:	5-Okt-2022
Erscheinungsdatum:	2015
Sprache des Dokuments:	Englisch
Zusammenfassung/Abstract:	The pathogenesis of glaucoma, a common neurodegenerative disease, involves an immunologic component. Changes in the natural autoantibody profile of glaucoma patients were detected, showing not only up-regulated but also down-regulated immunoreactivities. In recent studies we were able to demonstrate that the antibody changes have a large influence on protein profiles of neuroretinal cells. Furthermore we could demonstrate neuroprotective potential of one of the down-regulated antibodies (gamma-synuclein antibody). Anti-GFAP antibody is another antibody found down-regulated in glaucoma patients. Since GFAP expression is intensified in glaucomatous retina, the aim of this study was to detect the effect of GFAP antibodies on neuroretinal cells. This is realized with a viability-test as well as proteomic analysis of cells incubated with GFAP antibodies. Furthermore, possible interaction partners of the GFAP antibody in neuroretinal cells were identified by western blot, mass spectrometry and indirect immunofluorescence staining. We found that the GFAP antibody is able to protect cells from oxidative stress, which is due to changed protein expressions of the actin cytoskeleton. Furthermore we detected a cross-reaction of the antibody to endoplasmic reticulum resident protein 57 on the cell membrane, which seems to lead to a changed signaling in the cells triggering the protective effects.
DDC-Sachgruppe:	610 Medizin 610 Medical sciences
Veröffentlichende Institution:	Johannes Gutenberg-Universität Mainz
Organisationseinheit:	FB 04 Medizin
Veröffentlichungsort:	Mainz
ROR:	https://ror.org/023b0x485
DOI:	http://doi.org/10.25358/openscience-7853
Version:	Published version
Publikationstyp:	Zeitschriftenaufsatz
Nutzungsrechte:	CC BY-NC-ND
Informationen zu den Nutzungsrechten:	https://creativecommons.org/licenses/by-nc-nd/4.0/
Zeitschrift:	Journal of pharmacological sciences 127 3
Seitenzahl oder Artikelnummer:	298 304
Verlag:	The Japanese Pharmacological Society
Verlagsort:	Tokyo
Erscheinungsdatum:	2015
ISSN:	1347-8648 1347-8613
URL der Originalveröffentlichung:	http://dx.doi.org/10.1016/j.jphs.2014.12.019
DOI der Originalveröffentlichung:	10.1016/j.jphs.2014.12.019
Enthalten in den Sammlungen:	DFG-OA-Publizieren (2012 - 2017)