Gutenberg Open Science: GFAP antibodies show protective effect on oxidatively stressed neuroretinal cells via interaction with ERP57

Please use this identifier to cite or link to this item: http://doi.org/10.25358/openscience-7853

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DC Field	Value	Language
dc.contributor.author	Wilding, Corina	-
dc.contributor.author	Bell, Katharina	-
dc.contributor.author	Funke, S.	-
dc.contributor.author	Beck, Sabine	-
dc.contributor.author	Pfeiffer, Norbert	-
dc.contributor.author	Grus, Franz-Hermann	-
dc.date.accessioned	2022-10-05T09:27:18Z	-
dc.date.available	2022-10-05T09:27:18Z	-
dc.date.issued	2015	-
dc.identifier.uri	https://openscience.ub.uni-mainz.de/handle/20.500.12030/7868	-
dc.description.abstract	The pathogenesis of glaucoma, a common neurodegenerative disease, involves an immunologic component. Changes in the natural autoantibody profile of glaucoma patients were detected, showing not only up-regulated but also down-regulated immunoreactivities. In recent studies we were able to demonstrate that the antibody changes have a large influence on protein profiles of neuroretinal cells. Furthermore we could demonstrate neuroprotective potential of one of the down-regulated antibodies (gamma-synuclein antibody). Anti-GFAP antibody is another antibody found down-regulated in glaucoma patients. Since GFAP expression is intensified in glaucomatous retina, the aim of this study was to detect the effect of GFAP antibodies on neuroretinal cells. This is realized with a viability-test as well as proteomic analysis of cells incubated with GFAP antibodies. Furthermore, possible interaction partners of the GFAP antibody in neuroretinal cells were identified by western blot, mass spectrometry and indirect immunofluorescence staining. We found that the GFAP antibody is able to protect cells from oxidative stress, which is due to changed protein expressions of the actin cytoskeleton. Furthermore we detected a cross-reaction of the antibody to endoplasmic reticulum resident protein 57 on the cell membrane, which seems to lead to a changed signaling in the cells triggering the protective effects.	en_GB
dc.description.sponsorship	DFG, Open Access-Publizieren Universität Mainz / Universitätsmedizin	de
dc.language.iso	eng	de
dc.rights	CC BY-NC-ND	*
dc.rights.uri	https://creativecommons.org/licenses/by-nc-nd/4.0/	*
dc.subject.ddc	610 Medizin	de_DE
dc.subject.ddc	610 Medical sciences	en_GB
dc.title	GFAP antibodies show protective effect on oxidatively stressed neuroretinal cells via interaction with ERP57	en_GB
dc.type	Zeitschriftenaufsatz	de
dc.identifier.doi	http://doi.org/10.25358/openscience-7853	-
jgu.type.dinitype	article	en_GB
jgu.type.version	Published version	de
jgu.type.resource	Text	de
jgu.organisation.department	FB 04 Medizin	de
jgu.organisation.number	2700	-
jgu.organisation.name	Johannes Gutenberg-Universität Mainz	-
jgu.rights.accessrights	openAccess	-
jgu.journal.title	Journal of pharmacological sciences	de
jgu.journal.volume	127	de
jgu.journal.issue	3	de
jgu.pages.start	298	de
jgu.pages.end	304	de
jgu.publisher.year	2015	-
jgu.publisher.name	The Japanese Pharmacological Society	de
jgu.publisher.place	Tokyo	de
jgu.publisher.uri	http://dx.doi.org/10.1016/j.jphs.2014.12.019	de
jgu.publisher.issn	1347-8648	de
jgu.publisher.issn	1347-8613	de
jgu.organisation.place	Mainz	-
jgu.identifier.pmid	25837926	-
jgu.subject.ddccode	610	de
opus.date.modified	2018-09-05T09:15:51Z	-
opus.subject.dfgcode	00-000	-
opus.organisation.string	FB 04: Medizin: Augenklinik und Poliklinik	de_DE
opus.identifier.opusid	51986	-
opus.importsource	pubmed	-
opus.institute.number	0446	-
opus.metadataonly	false	-
opus.type.contenttype	Keine	de_DE
opus.type.contenttype	None	en_EN
opus.affiliated	Pfeiffer, Norbert	-
opus.affiliated	Grus, Franz-Hermann	-
jgu.publisher.doi	10.1016/j.jphs.2014.12.019	de
jgu.organisation.ror	https://ror.org/023b0x485	-
Appears in collections:	DFG-OA-Publizieren (2012 - 2017)

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