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Authors: Righesso, Leonardo A. R.
Terekhov, Maxim
Götz, Hermann
Ackermann, Maximilian
Emrich, Tilman
Schreiber, Laura Maria
Müller, W. E. G.
Jung, J.
Rojas, Javier Patricio
Al-Nawas, Bilal
Title: Dynamic contrast-enhanced magnetic resonance imaging for monitoring neovascularization during bone regeneration : a randomized in vivo study in rabbits
Online publication date: 15-Aug-2022
Year of first publication: 2021
Language: english
Abstract: Objectives Micro-computed tomography (μ-CT) and histology, the current gold standard methods for assessing the formation of new bone and blood vessels, are invasive and/or destructive. With that in mind, a more conservative tool, dynamic contrast-enhanced magnetic resonance imaging (DCE-MRI), was tested for its accuracy and reproducibility in monitoring neovascularization during bone regeneration. Additionally, the suitability of blood perfusion as a surrogate of the efficacy of osteoplastic materials was evaluated. Materials and methods Sixteen rabbits were used and equally divided into four groups, according to the time of euthanasia (2, 3, 4, and 6 weeks after surgery). The animals were submitted to two 8-mm craniotomies that were filled with blood or autogenous bone. Neovascularization was assessed in vivo through DCE-MRI, and bone regeneration, ex vivo, through μ-CT and histology. Results The defects could be consistently identified, and their blood perfusion measured through DCE-MRI, there being statistically significant differences within the blood clot group between 3 and 6 weeks (p = 0.029), and between the former and autogenous bone at six weeks (p = 0.017). Nonetheless, no significant correlations between DCE-MRI findings on neovascularization and μ-CT (r =−0.101, 95% CI [−0.445; 0.268]) or histology (r = 0.305, 95% CI [−0.133; 0.644]) findings on bone regeneration were observed. Conclusions These results support the hypothesis that DCE-MRI can be used to monitor neovascularization but contradict the premise that it could predict bone regeneration as well.
DDC: 610 Medizin
610 Medical sciences
Institution: Johannes Gutenberg-Universität Mainz
Department: FB 04 Medizin
Place: Mainz
Version: Published version
Publication type: Zeitschriftenaufsatz
License: CC BY
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Journal: Clinical oral investigations
Pages or article number: 5843
Publisher: Springer
Publisher place: Berlin u.a.
Issue date: 2021
ISSN: 1436-3771
Publisher DOI: 10.1007/s00784-021-03889-6
Appears in collections:JGU-Publikationen

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