Autoren:	Volz, Ann-Kathrin Frei, Alina Kretschmer, Viola Jesus Domingues, António M. de Ketting, Rene F. Ueffing, Marius Boldt, Karsten Krämer-Albers, Eva-Maria May-Simera, Helen L.
Titel:	Bardet-Biedl syndrome proteins modulate the release of bioactive extracellular vesicles
Online-Publikationsdatum:	4-Jul-2022
Erscheinungsdatum:	2021
Sprache des Dokuments:	Englisch
Zusammenfassung/Abstract:	Primary cilia are microtubule based sensory organelles important for receiving and processing cellular signals. Recent studies have shown that cilia also release extracellular vesicles (EVs). Because EVs have been shown to exert various physiological functions, these findings have the potential to alter our understanding of how primary cilia regulate specific signalling pathways. So far the focus has been on lgEVs budding directly from the ciliary membrane. An association between cilia and MVB-derived smEVs has not yet been described. We show that ciliary mutant mammalian cells demonstrate increased secretion of small EVs (smEVs) and a change in EV composition. Characterisation of smEV cargo identified signalling molecules that are differentially loaded upon ciliary dysfunction. Furthermore, we show that these smEVs are biologically active and modulate the WNT response in recipient cells. These results provide us with insights into smEV-dependent ciliary signalling mechanisms which might underly ciliopathy disease pathogenesis.
DDC-Sachgruppe:	570 Biowissenschaften 570 Life sciences
Veröffentlichende Institution:	Johannes Gutenberg-Universität Mainz
Organisationseinheit:	FB 10 Biologie
Veröffentlichungsort:	Mainz
ROR:	https://ror.org/023b0x485
DOI:	http://doi.org/10.25358/openscience-7299
Version:	Published version
Publikationstyp:	Zeitschriftenaufsatz
Nutzungsrechte:	CC BY
Informationen zu den Nutzungsrechten:	https://creativecommons.org/licenses/by/4.0/
Zeitschrift:	Nature Communications 12
Seitenzahl oder Artikelnummer:	5671
Verlag:	Nature Publishing Group
Verlagsort:	London
Erscheinungsdatum:	2021
ISSN:	2041-1723
DOI der Originalveröffentlichung:	10.1038/s41467-021-25929-1
Enthalten in den Sammlungen:	JGU-Publikationen