Please use this identifier to cite or link to this item:
http://doi.org/10.25358/openscience-7299
Authors: | Volz, Ann-Kathrin Frei, Alina Kretschmer, Viola Jesus Domingues, António M. de Ketting, Rene F. Ueffing, Marius Boldt, Karsten Krämer-Albers, Eva-Maria May-Simera, Helen L. |
Title: | Bardet-Biedl syndrome proteins modulate the release of bioactive extracellular vesicles |
Online publication date: | 4-Jul-2022 |
Year of first publication: | 2021 |
Language: | english |
Abstract: | Primary cilia are microtubule based sensory organelles important for receiving and processing cellular signals. Recent studies have shown that cilia also release extracellular vesicles (EVs). Because EVs have been shown to exert various physiological functions, these findings have the potential to alter our understanding of how primary cilia regulate specific signalling pathways. So far the focus has been on lgEVs budding directly from the ciliary membrane. An association between cilia and MVB-derived smEVs has not yet been described. We show that ciliary mutant mammalian cells demonstrate increased secretion of small EVs (smEVs) and a change in EV composition. Characterisation of smEV cargo identified signalling molecules that are differentially loaded upon ciliary dysfunction. Furthermore, we show that these smEVs are biologically active and modulate the WNT response in recipient cells. These results provide us with insights into smEV-dependent ciliary signalling mechanisms which might underly ciliopathy disease pathogenesis. |
DDC: | 570 Biowissenschaften 570 Life sciences |
Institution: | Johannes Gutenberg-Universität Mainz |
Department: | FB 10 Biologie |
Place: | Mainz |
ROR: | https://ror.org/023b0x485 |
DOI: | http://doi.org/10.25358/openscience-7299 |
Version: | Published version |
Publication type: | Zeitschriftenaufsatz |
License: | CC BY |
Information on rights of use: | https://creativecommons.org/licenses/by/4.0/ |
Journal: | Nature Communications 12 |
Pages or article number: | 5671 |
Publisher: | Nature Publishing Group |
Publisher place: | London |
Issue date: | 2021 |
ISSN: | 2041-1723 |
Publisher DOI: | 10.1038/s41467-021-25929-1 |
Appears in collections: | JGU-Publikationen |
Files in This Item:
File | Description | Size | Format | ||
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bardetbiedl_syndrome_proteins-20220704112638139.pdf | 4.78 MB | Adobe PDF | View/Open |