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Authors: Volz, Ann-Kathrin
Frei, Alina
Kretschmer, Viola
Jesus Domingues, António M. de
Ketting, Rene F.
Ueffing, Marius
Boldt, Karsten
Krämer-Albers, Eva-Maria
May-Simera, Helen L.
Title: Bardet-Biedl syndrome proteins modulate the release of bioactive extracellular vesicles
Online publication date: 4-Jul-2022
Language: english
Abstract: Primary cilia are microtubule based sensory organelles important for receiving and processing cellular signals. Recent studies have shown that cilia also release extracellular vesicles (EVs). Because EVs have been shown to exert various physiological functions, these findings have the potential to alter our understanding of how primary cilia regulate specific signalling pathways. So far the focus has been on lgEVs budding directly from the ciliary membrane. An association between cilia and MVB-derived smEVs has not yet been described. We show that ciliary mutant mammalian cells demonstrate increased secretion of small EVs (smEVs) and a change in EV composition. Characterisation of smEV cargo identified signalling molecules that are differentially loaded upon ciliary dysfunction. Furthermore, we show that these smEVs are biologically active and modulate the WNT response in recipient cells. These results provide us with insights into smEV-dependent ciliary signalling mechanisms which might underly ciliopathy disease pathogenesis.
DDC: 570 Biowissenschaften
570 Life sciences
Institution: Johannes Gutenberg-Universität Mainz
Department: FB 10 Biologie
Place: Mainz
Version: Published version
Publication type: Zeitschriftenaufsatz
License: CC BY
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Journal: Nature Communications
Pages or article number: 5671
Publisher: Nature Publishing Group
Publisher place: London
Issue date: 2021
ISSN: 2041-1723
Publisher DOI: 10.1038/s41467-021-25929-1
Appears in collections:JGU-Publikationen

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