Please use this identifier to cite or link to this item:
http://doi.org/10.25358/openscience-7274
Authors: | Riegel, Kristina Yurugi, Hajime Schlöder, Janine Jonuleit, Helmut Kaulich, Manuel Kirschner, Friederike Arnold-Schild, Danielle Tenzer, Stefan Schild, Hansjörg Rajalingam, Krishnaraj |
Title: | ERK5 modulates IL-6 secretion and contributes to tumor-induced immune suppression |
Online publication date: | 1-Jul-2022 |
Year of first publication: | 2021 |
Language: | english |
Abstract: | Tumors exhibit a variety of strategies to dampen antitumor immune responses. With an aim to identify factors that are secreted from tumor cells, we performed an unbiased mass spectrometry-based secretome analysis in lung cancer cells. Interleukin-6 (IL-6) has been identified as a prominent factor secreted by tumor cells and cancer-associated fibroblasts isolated from cancer patients. Incubation of dendritic cell (DC) cultures with tumor cell supernatants inhibited the production of IL-12p70 in DCs but not the surface expression of other activation markers which is reversed by treatment with IL-6 antibody. Defects in IL-12p70 production in the DCs inhibited the differentiation of Th1 but not Th2 and Th17 cells from naïve CD4+ T cells. We also demonstrate that the classical mitogen-activated protein kinase, ERK5/MAPK7, is required for IL-6 production in tumor cells. Inhibition of ERK5 activity or depletion of ERK5 prevented IL-6 production in tumor cells, which could be exploited for enhancing antitumor immune responses. |
DDC: | 610 Medizin 610 Medical sciences |
Institution: | Johannes Gutenberg-Universität Mainz |
Department: | FB 04 Medizin |
Place: | Mainz |
ROR: | https://ror.org/023b0x485 |
DOI: | http://doi.org/10.25358/openscience-7274 |
Version: | Published version |
Publication type: | Zeitschriftenaufsatz |
License: | CC BY |
Information on rights of use: | https://creativecommons.org/licenses/by/4.0/ |
Journal: | Cell death & disease 12 |
Pages or article number: | 969 |
Publisher: | Nature Publishing Group |
Publisher place: | London u.a. |
Issue date: | 2021 |
ISSN: | 2041-4889 |
Publisher DOI: | 10.1038/s41419-021-04257-8 |
Appears in collections: | JGU-Publikationen |
Files in This Item:
File | Description | Size | Format | ||
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![]() | erk5_modulates_il6_secretion_-20220701105326466.pdf | 1.61 MB | Adobe PDF | View/Open |