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http://doi.org/10.25358/openscience-7269| Authors: | Deng, Jie Walther, Andreas |
| Title: | Autonomous DNA nanostructures instructed by hierarchically concatenated chemical reaction networks |
| Online publication date: | 1-Jul-2022 |
| Year of first publication: | 2021 |
| Language: | english |
| Abstract: | Concatenation and communication between chemically distinct chemical reaction networks (CRNs) is an essential principle in biology for controlling dynamics of hierarchical structures. Here, to provide a model system for such biological systems, we demonstrate autonomous lifecycles of DNA nanotubes (DNTs) by two concatenated CRNs using different thermodynamic principles: (1) ATP-powered ligation/restriction of DNA components and (2) input strand-mediated DNA strand displacement (DSD) using energy gains provided in DNA toeholds. This allows to achieve hierarchical non-equilibrium systems by concurrent ATP-powered ligation-induced DSD for activating DNT self-assembly and restriction-induced backward DSD reactions for triggering DNT degradation. We introduce indirect and direct activation of DNT self-assemblies, and orthogonal molecular recognition allows ATP-fueled self-sorting of transient multicomponent DNTs. Coupling ATP dissipation to DNA nanostructures via programmable DSD is a generic concept which should be widely applicable to organize other DNA nanostructures, and enable the design of automatons and life-like systems of higher structural complexity. |
| DDC: | 540 Chemie 540 Chemistry and allied sciences 570 Biowissenschaften 570 Life sciences |
| Institution: | Johannes Gutenberg-Universität Mainz |
| Department: | FB 09 Chemie, Pharmazie u. Geowissensch. |
| Place: | Mainz |
| ROR: | https://ror.org/023b0x485 |
| DOI: | http://doi.org/10.25358/openscience-7269 |
| Version: | Published version |
| Publication type: | Zeitschriftenaufsatz |
| License: | CC BY |
| Information on rights of use: | https://creativecommons.org/licenses/by/4.0/ |
| Journal: | Nature Communications 12 |
| Pages or article number: | 5132 |
| Publisher: | Nature Publishing Group UK |
| Publisher place: | London |
| Issue date: | 2021 |
| ISSN: | 2041-1723 |
| Publisher DOI: | 10.1038/s41467-021-25450-5 |
| Appears in collections: | JGU-Publikationen |
Files in This Item:
| File | Description | Size | Format | ||
|---|---|---|---|---|---|
 | autonomous_dna_nanostructures-20220701085546809.pdf | 7.05 MB | Adobe PDF | View/Open |