Please use this identifier to cite or link to this item:
Authors: Deng, Jie
Walther, Andreas
Title: Autonomous DNA nanostructures instructed by hierarchically concatenated chemical reaction networks
Online publication date: 1-Jul-2022
Year of first publication: 2021
Language: english
Abstract: Concatenation and communication between chemically distinct chemical reaction networks (CRNs) is an essential principle in biology for controlling dynamics of hierarchical structures. Here, to provide a model system for such biological systems, we demonstrate autonomous lifecycles of DNA nanotubes (DNTs) by two concatenated CRNs using different thermodynamic principles: (1) ATP-powered ligation/restriction of DNA components and (2) input strand-mediated DNA strand displacement (DSD) using energy gains provided in DNA toeholds. This allows to achieve hierarchical non-equilibrium systems by concurrent ATP-powered ligation-induced DSD for activating DNT self-assembly and restriction-induced backward DSD reactions for triggering DNT degradation. We introduce indirect and direct activation of DNT self-assemblies, and orthogonal molecular recognition allows ATP-fueled self-sorting of transient multicomponent DNTs. Coupling ATP dissipation to DNA nanostructures via programmable DSD is a generic concept which should be widely applicable to organize other DNA nanostructures, and enable the design of automatons and life-like systems of higher structural complexity.
DDC: 540 Chemie
540 Chemistry and allied sciences
570 Biowissenschaften
570 Life sciences
Institution: Johannes Gutenberg-Universität Mainz
Department: FB 09 Chemie, Pharmazie u. Geowissensch.
Place: Mainz
Version: Published version
Publication type: Zeitschriftenaufsatz
License: CC BY
Information on rights of use:
Journal: Nature Communications
Pages or article number: 5132
Publisher: Nature Publishing Group UK
Publisher place: London
Issue date: 2021
ISSN: 2041-1723
Publisher DOI: 10.1038/s41467-021-25450-5
Appears in collections:JGU-Publikationen

Files in This Item:
  File Description SizeFormat
autonomous_dna_nanostructures-20220701085546809.pdf7.05 MBAdobe PDFView/Open