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Authors: Dietz, Sabrina
Vasconcelos Almeida, Miguel
Nischwitz, Emily
Schreier, Jan
Viceconte, Nikenza
Fradera-Sola, Albert
Renz, Christian
Ceron-Noriega, Alejandro
Ulrich, Helle D.
Kappei, Dennis
Ketting, René F.
Butter, Falk
Title: The double-stranded DNA-binding proteins TEBP-1 and TEBP-2 form a telomeric complex with POT-1
Online publication date: 17-May-2022
Language: english
Abstract: Telomeres are bound by dedicated proteins, which protect them from DNA damage and regulate telomere length homeostasis. In the nematode Caenorhabditis elegans, a comprehensive understanding of the proteins interacting with the telomere sequence is lacking. Here, we harnessed a quantitative proteomics approach to identify TEBP-1 and TEBP-2, two paralogs expressed in the germline and embryogenesis that associate to telomeres in vitro and in vivo. tebp-1 and tebp-2 mutants display strikingly distinct phenotypes: tebp-1 mutants have longer telomeres than wild-type animals, while tebp-2 mutants display shorter telomeres and a Mortal Germline. Notably, tebp-1;tebp-2 double mutant animals have synthetic sterility, with germlines showing signs of severe mitotic and meiotic arrest. Furthermore, we show that POT-1 forms a telomeric complex with TEBP-1 and TEBP-2, which bridges TEBP-1/-2 with POT-2/MRT-1. These results provide insights into the composition and organization of a telomeric protein complex in C. elegans.
DDC: 570 Biowissenschaften
570 Life sciences
Institution: Johannes Gutenberg-Universität Mainz
Department: Sonderforschungsbereiche (SFB)
Place: Mainz
Version: Published version
Publication type: Zeitschriftenaufsatz
License: CC BY
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Journal: Nature Communications
Pages or article number: 2668
Publisher: Nature Publishing Group UK
Publisher place: London
Issue date: 2021
ISSN: 2041-1723
Publisher DOI: 10.1038/s41467-021-22861-2
Appears in collections:JGU-Publikationen

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