Please use this identifier to cite or link to this item:
http://doi.org/10.25358/openscience-6986
Authors: | Dietz, Sabrina Vasconcelos Almeida, Miguel Nischwitz, Emily Schreier, Jan Viceconte, Nikenza Fradera-Sola, Albert Renz, Christian Ceron-Noriega, Alejandro Ulrich, Helle D. Kappei, Dennis Ketting, René F. Butter, Falk |
Title: | The double-stranded DNA-binding proteins TEBP-1 and TEBP-2 form a telomeric complex with POT-1 |
Online publication date: | 17-May-2022 |
Year of first publication: | 2021 |
Language: | english |
Abstract: | Telomeres are bound by dedicated proteins, which protect them from DNA damage and regulate telomere length homeostasis. In the nematode Caenorhabditis elegans, a comprehensive understanding of the proteins interacting with the telomere sequence is lacking. Here, we harnessed a quantitative proteomics approach to identify TEBP-1 and TEBP-2, two paralogs expressed in the germline and embryogenesis that associate to telomeres in vitro and in vivo. tebp-1 and tebp-2 mutants display strikingly distinct phenotypes: tebp-1 mutants have longer telomeres than wild-type animals, while tebp-2 mutants display shorter telomeres and a Mortal Germline. Notably, tebp-1;tebp-2 double mutant animals have synthetic sterility, with germlines showing signs of severe mitotic and meiotic arrest. Furthermore, we show that POT-1 forms a telomeric complex with TEBP-1 and TEBP-2, which bridges TEBP-1/-2 with POT-2/MRT-1. These results provide insights into the composition and organization of a telomeric protein complex in C. elegans. |
DDC: | 570 Biowissenschaften 570 Life sciences |
Institution: | Johannes Gutenberg-Universität Mainz |
Department: | Sonderforschungsbereiche (SFB) |
Place: | Mainz |
ROR: | https://ror.org/023b0x485 |
DOI: | http://doi.org/10.25358/openscience-6986 |
Version: | Published version |
Publication type: | Zeitschriftenaufsatz |
License: | CC BY |
Information on rights of use: | https://creativecommons.org/licenses/by/4.0/ |
Journal: | Nature Communications 12 |
Pages or article number: | 2668 |
Publisher: | Nature Publishing Group UK |
Publisher place: | London |
Issue date: | 2021 |
ISSN: | 2041-1723 |
Publisher DOI: | 10.1038/s41467-021-22861-2 |
Appears in collections: | JGU-Publikationen |
Files in This Item:
File | Description | Size | Format | ||
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the_doublestranded_dnabinding-20220517111834474.pdf | 7.15 MB | Adobe PDF | View/Open |