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Authors: Hess, Cornelius
Zeidler, Sandra
Röhrich, Jörg
Title: Forensic application and evaluation of a commercially available pregabalin immunoassay test in serum on an Olympus AU480
Online publication date: 27-Apr-2022
Year of first publication: 2021
Language: english
Abstract: Misuse of pregabalin and its forensic relevance is steadily increasing. The aim of this study was to evaluate the usability of the commercially available ARKTM Pregabalin II Assay (ARK Diagnostics) for serum analysis of forensic samples. Overall, 156 samples were tested by both the immunoassay and a validated liquid chromatographic–tandem mass spectrometric (LC-MS/MS) method. Sensitivity was 100%, and specificity was 98.7% (n = 79 positive cases confirmed by LC-MS/MS in a range of 380–37,000 ng/mL). A good correlation (R2 = 0.73) could also be shown between quantitative immunoassay and LC-MS/MS results. In conclusion, the assay shows excellent reliability for screening of forensic serum samples.
DDC: 610 Medizin
610 Medical sciences
Institution: Johannes Gutenberg-Universität Mainz
Department: FB 04 Medizin
Place: Mainz
Version: Published version
Publication type: Zeitschriftenaufsatz
License: CC BY
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Journal: Drug testing and analysis
Pages or article number: 1216
Publisher: Wiley
Publisher place: Hoboken, NJ
Issue date: 2021
ISSN: 1942-7611
Publisher DOI: 10.1002/dta.2994
Appears in collections:JGU-Publikationen

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