Autoren:	Hess, Cornelius Zeidler, Sandra Röhrich, Jörg
Titel:	Forensic application and evaluation of a commercially available pregabalin immunoassay test in serum on an Olympus AU480
Online-Publikationsdatum:	27-Apr-2022
Erscheinungsdatum:	2021
Sprache des Dokuments:	Englisch
Zusammenfassung/Abstract:	Misuse of pregabalin and its forensic relevance is steadily increasing. The aim of this study was to evaluate the usability of the commercially available ARKTM Pregabalin II Assay (ARK Diagnostics) for serum analysis of forensic samples. Overall, 156 samples were tested by both the immunoassay and a validated liquid chromatographic–tandem mass spectrometric (LC-MS/MS) method. Sensitivity was 100%, and specificity was 98.7% (n = 79 positive cases confirmed by LC-MS/MS in a range of 380–37,000 ng/mL). A good correlation (R2 = 0.73) could also be shown between quantitative immunoassay and LC-MS/MS results. In conclusion, the assay shows excellent reliability for screening of forensic serum samples.
DDC-Sachgruppe:	610 Medizin 610 Medical sciences
Veröffentlichende Institution:	Johannes Gutenberg-Universität Mainz
Organisationseinheit:	FB 04 Medizin
Veröffentlichungsort:	Mainz
ROR:	https://ror.org/023b0x485
DOI:	http://doi.org/10.25358/openscience-6912
Version:	Published version
Publikationstyp:	Zeitschriftenaufsatz
Nutzungsrechte:	CC BY
Informationen zu den Nutzungsrechten:	https://creativecommons.org/licenses/by/4.0/
Zeitschrift:	Drug testing and analysis 13 6
Seitenzahl oder Artikelnummer:	1216 1218
Verlag:	Wiley
Verlagsort:	Hoboken, NJ
Erscheinungsdatum:	2021
ISSN:	1942-7611
DOI der Originalveröffentlichung:	10.1002/dta.2994
Enthalten in den Sammlungen:	JGU-Publikationen