Please use this identifier to cite or link to this item: http://doi.org/10.25358/openscience-6699
Authors: Gribko, Alena
Stiefel, Janis
Liebetanz, Lana
Nagel, Sophie Madeleine
Künzel, Julian
Wandrey, Madita
Hagemann, Jan
Stauber, Roland H.
Freese, Christian
Gül, Désirée
Title: IsoMAG: an automated system for the immunomagnetic isolation of squamous cell carcinoma-derived dirculating tumor dells
Online publication date: 14-Jan-2022
Language: english
Abstract: Background: detailed information about circulating tumor cells (CTCs) as an indicator of therapy response and cancer metastasis is crucial not only for basic research but also for diagnostics and therapeutic approaches. Here, we showcase a newly developed IsoMAG IMS system with an optimized protocol for fully automated immunomagnetic enrichment of CTCs, also revealing rare CTC subpopulations. Methods: using different squamous cell carcinoma cell lines, we developed an isolation protocol exploiting highly efficient EpCAM-targeting magnetic beads for automated CTC enrichment by the IsoMAG IMS system. By FACS analysis, we analyzed white blood contamination usually preventing further downstream analysis of enriched cells. Results: 1 µm magnetic beads with tosyl-activated hydrophobic surface properties were found to be optimal for automated CTC enrichment. More than 86.5% and 95% of spiked cancer cells were recovered from both cell culture media or human blood employing our developed protocol. In addition, contamination with white blood cells was minimized to about 1200 cells starting from 7.5 mL blood. Finally, we showed that the system is applicable for HNSCC patient samples and characterized isolated CTCs by immunostaining using a panel of tumor markers. Conclusion: Herein, we demonstrate that the IsoMAG system allows the detection and isolation of CTCs from HNSCC patient blood for disease monitoring in a fully-automated process with a significant leukocyte count reduction. Future developments seek to integrate the IsoMAG IMS system into an automated microfluidic-based isolation workflow to further facilitate single CTC detection also in clinical routine.
DDC: 610 Medizin
610 Medical sciences
Institution: Johannes Gutenberg-Universität Mainz
Department: FB 04 Medizin
Place: Mainz
DOI: http://doi.org/10.25358/openscience-6699
Version: Published version
Publication type: Zeitschriftenaufsatz
Document type specification: Scientific article
License: CC BY
Information on rights of use: https://creativecommons.org/licenses/by/4.0/
Journal: Diagnostics
11
11
Pages or article number: 2040
Publisher: MDPI
Publisher place: Basel
Issue date: 2021
ISSN: 2075-4418
Publisher URL: https://doi.org/10.3390/diagnostics11112040
Publisher DOI: 10.3390/diagnostics11112040
Appears in collections:JGU-Publikationen

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