Authors:	Rauhala, Onni J. Dominguez, Soledad Spyropoulos, George D. Ferrero, Jose Javier Boyers, Talia R. Jastrzebska-Perfect, Patricia Cea, Claudia Khodagholy, Dion Gelinas, Jennifer N.
Title:	Chitosan-based, biocompatible, solution processable films for in vivo localization of neural interface devices
Online publication date:	25-Oct-2021
Year of first publication:	2020
Language:	english
Abstract:	Chitosan (CS) is a biocompatible, inexpensive organic polymer that is increasingly used in neural tissue applications. However, its intrinsic fluorescence has not yet been leveraged to facilitate localization of neural interface devices, a key procedure to ensure accurate analysis of neurophysiological signals. A process is developed to enable control of mechanical and chemical properties of CS-based composites, generating freestanding films that are stable in aqueous environments and exhibit concentration-dependent fluorescence intensity. The shape and location of CS-coated probes are reliably visualized in vitro and in vivo using fluorescence microscopy. Furthermore, CS neural probe marking is fully compatible with classical immunohistochemical and histological techniques, enabling localization of high spatiotemporal resolution surface electrocorticography arrays in adult rats and mouse pups. CS composites have the potential to simplify and streamline experimental procedures required to efficiently acquire, localize, and interpret neurophysiological data.
DDC:	570 Biowissenschaften 570 Life sciences 600 Technik 600 Technology (Applied sciences) 610 Medizin 610 Medical sciences 620 Ingenieurwissenschaften und Maschinenbau 620 Engineering and allied operations 621.3 Elektrotechnik 621.3 Electric engineering
Institution:	Johannes Gutenberg-Universität Mainz
Department:	FB 04 Medizin
Place:	Mainz
ROR:	https://ror.org/023b0x485
DOI:	http://doi.org/10.25358/openscience-6401
Version:	Published version
Publication type:	Zeitschriftenaufsatz
Document type specification:	Scientific article
License:	CC BY-NC
Information on rights of use:	https://creativecommons.org/licenses/by-nc/4.0/
Journal:	Advanced materials technologies 5 3
Pages or article number:	1900663
Publisher:	Wiley
Publisher place:	Weinheim
Issue date:	2020
ISSN:	2365-709X
Publisher URL:	https://doi.org/10.1002/admt.201900663
Publisher DOI:	10.1002/admt.201900663
Appears in collections:	JGU-Publikationen